Human Carbonic Anhydrase 3(CA3) ELISA Kit

Human Carbonic Anhydrase 3(CA3) ELISA Kit

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中文名稱：

人碳酸酐酶3(CA3)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E15962h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人碳酸酐酶3(CA3)酶聯(lián)免疫試劑盒（CSB-E15962h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CA3含量。CA3 作為一種靶點(diǎn)，在神經(jīng)科學(xué)等領(lǐng)域有重要研究價(jià)值。它是大腦海馬體的重要組成部分，在學(xué)習(xí)、記憶等認(rèn)知功能中起關(guān)鍵作用。研究機(jī)制主要圍繞其神經(jīng)環(huán)路、信號(hào)傳導(dǎo)及相關(guān)分子機(jī)制，以深入了解其功能及在疾病中的變化。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，適用于肌肉生物學(xué)研究、代謝性疾病模型分析、運(yùn)動(dòng)生理學(xué)評(píng)估等科研領(lǐng)域，為探索CA3在能量代謝、肌肉功能調(diào)控及相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CA III ELISA Kit; CA-III ELISA Kit; Ca3 ELISA Kit; CAH3_HUMAN ELISA Kit; CAIII ELISA Kit; Car3 ELISA Kit; Carbonate dehydratase III ELISA Kit; Carbonic anhydrase 3 ELISA Kit; Carbonic anhydrase III ELISA Kit; Carbonic anhydrase III muscle specific ELISA Kit; EC 4.2.1.1 ELISA Kit; Epididymis secretory sperm binding protein Li 167mP ELISA Kit; HEL S 167mP ELISA Kit
縮寫：

CA3
Uniprot No.：

P07451
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cell Biology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	95
	Range %	90-105
1:4	Average %	103
	Range %	97-107
1:8	Average %	89
	Range %	85-95

回收率：

The recovery of human CA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.748	2.579	2.664	2.512
250	2.187	2.136	2.162	2.010
125	1.467	1.488	1.478	1.326
62.5	0.897	0.722	0.810	0.658
31.2	0.488	0.490	0.489	0.337
15.6	0.346	0.345	0.346	0.194
7.8	0.225	0.237	0.231	0.079
0	0.156	0.148	0.152

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Aldolase A and carbonic anhydrase III, two new candidate biomarkers versus acknowledged parameters in the context of crushing syndrome IC CREANG?,Romanian Journal of Medical Practice,2021

功能：

Reversible hydration of carbon dioxide.
基因功能參考文獻(xiàn)：
1. CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis. PMID: 26847302
2. this study detected the formation and colocalization of 6-nitrotryptophan-containing proteins and CAIII in the skin of atopic dermatitis patients, not only in the lesional part but also in the nonlesional part, through histochemical analyses PMID: 24838180
3. Report that anti-CAIII antibodies may be useful for diagnosing microscopic polyangiitis. PMID: 23981757
4. The carbonic anhydrase CA3 isoform displayed an increased abundance during muscle aging, which was independently verified by immunoblotting of differently aged human skeletal muscle samples. PMID: 22797148
5. The generation of CA III and IV autoantibodies, antioxidant enzymes, and cytokines might influence each other. PMID: 23049597
6. Crystallization and preliminary X-ray analysis of human carbonic anhydrase III PMID: 11976500
7. Data show that inserting histidine residues into the active site cavity of carbonic anhydrase II or III results in rates of proton transfer to the zinc-bound hydroxide that are antagonistic or suppressive with respect to the corresponding single mutants. PMID: 12171926
8. Proton transfer within the active-site cavity of carbonic anhydrase III PMID: 12484342
9. myoglobin/carbonic anhydrase III ratio in the blood proved to be a more specific indicator for myocardial damage than myoglobin alone after myocardial infarction. PMID: 12745799
10. CAIII expression is upregulated in kidney cortex samples from the end-stage kidney of a patient with Dent's disease owing to the G506E mutation of CLCN5 PMID: 18322545
11. CAIII promotes transformation and invasion capability in hepatoma cells through FAK signaling pathway. PMID: 18444244
12. The level of CAIII is specifically insufficient in the skeletal muscle of myasthenia gravis patients. PMID: 19301202
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Alpha-carbonic anhydrase family
組織特異性：

Muscle specific.
數(shù)據(jù)庫鏈接：

HGNC: 1374

OMIM: 114750

KEGG: hsa:761

STRING: 9606.ENSP00000285381

UniGene: Hs.82129

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.