Human Bleomycin hydrolase(BLMH) ELISA kit

Human Bleomycin hydrolase(BLMH) ELISA kit

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中文名稱：

人博來霉素水解酶(BLMH)酶聯免疫試劑盒
貨號：

CSB-EL002716HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人博來霉素水解酶(BLMH)酶聯免疫試劑盒（CSB-EL002716HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BLMH含量。BLMH（博萊霉素水解酶）是一種在進化中高度保守的胞質半胱氨酸肽酶，主要功能是代謝失活抗腫瘤藥物博萊霉素（BLM），保護細胞免受其毒性作用。其研究機制涉及對BLM的羧酰胺鍵進行水解，維持細胞平衡和防御有害物質。試劑盒檢測范圍為28 pg/mL-1800 pg/mL，適用于體外培養細胞模型、動物組織樣本或生物體液中的BLMH蛋白定量分析，為探索其在細胞應激調控、藥物代謝機制研究、疾病相關分子標志物篩選等科研方向提供精準檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BH ELISA Kit; Bleomycin hydrolase ELISA Kit; BLM hydrolase ELISA Kit; BLMH ELISA Kit; BLMH_HUMAN ELISA Kit; BMH ELISA Kit; EC 3.4.22.40 ELISA Kit
縮寫：

BLMH
Uniprot No.：

Q13867
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

28 pg/mL-1800 pg/mL
靈敏度：

7 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BLMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	110
	Range %	107-113
1:2	Average %	89
	Range %	84-93
1:4	Average %	85
	Range %	81-89
1:8	Average %	86
	Range %	83-89

回收率：

The recovery of human BLMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-99
EDTA plasma (n=4)	105	101-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.364	2.329	2.347	2.225
900	1.526	1.568	1.547	1.425
450	0.961	0.975	0.968	0.846
225	0.592	0.578	0.585	0.463
112.5	0.383	0.372	0.378	0.256
56	0.298	0.271	0.285	0.163
28	0.197	0.189	0.193	0.071
0	0.121	0.123	0.122

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Bisbenzylisoquinoline Alkaloid Fangchinoline Derivative HY-2 Inhibits Breast Cancer Cells by Targeting BLM DNA Helicase W Zhang,SSRN,2023
Bovine colostrum induces the differentiation of human primary keratinocytes D Kovacs,FASEB J,2020
Identification of Potential Plasma Biomarkers for Abdominal Aortic Aneurysm Using Tandem Mass Tag Quantitative Proteomics Henriksson AE.et al,Proteomes,2018

功能：

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
基因功能參考文獻：
1. the BLMH gene single nucleotide polymorphism A1450G (rs1050565) influences BLMH activity and late pulmonary toxicity. PMID: 27327270
2. Ubc9 plays different roles of action in antitumor agents in chemotherapy. The process requires bleomycin hydrolase and poly(ADP-ribose) polymerase-1. The results are beneficial to deeply understanding of Ubc9 functions and for precise prediction of chemotherapy outcomes in tumors. PMID: 27878232
3. Bleomycin hydrolase downregulation in lesional skin of adult atopic dermatitis patients is independent of filaggrin gene mutations PMID: 25240784
4. This study findings suggest that Blmh interacts with diverse cellular processes from energy metabolism and anti-oxidative defenses to cell cycle, cytoskeleton dynamics, and synaptic plasticity essential for normal brain homeostasis. PMID: 24496069
5. We also detected significant association between XRCC1, XRCC3, and BLHX polymorphisms and a high frequency of chromosomal damage PMID: 24615029
6. The caspase-dependent cleavage of BLH was confirmed by cleavage of partly-purified human bleomycin hydrolase with caspase-3. PMID: 23708668
7. BH may play an important role during the late stage of epidermal differentiation. PMID: 22037625
8. present study suggests that our new method can detect novel genes of interest and that BLMH is a suppressor gene in HCC PMID: 21943823
9. This first report on BLMH carrier status in Tunisia shows o association between carrying the BLMH-G genotype and Alzheimer's disease in epsilon4 negative or positive subjects. PMID: 20198498
10. Cysteine proteases bleomycin hydrolase and cathepsin Z mediate N-terminal proteolysis and toxicity of mutant huntingtin. PMID: 21310951
11. BH activity and expression were markedly decreased in AD lesional skin, suggesting a defect of the filaggrin degradation pathway in AD. PMID: 21190945
12. Polymorphism is associated with neurodegenerative diseases, notably Alzheimer disease. PMID: 12604387
13. Significant effect of BLHX variant alleles (A/G, G/G) on the chromosome damage induced by bleomycin. PMID: 18082847
14. The homozygous variant G/G of BLMH gene SNP A1450G is associated with reduced survival and higher prevalence of early relapses in TC patients treated with bleomycin-containing chemotherapy. PMID: 18398146
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亞細胞定位：

Cytoplasm. Cytoplasmic granule.
蛋白家族：

Peptidase C1 family
數據庫鏈接：

HGNC: 1059

OMIM: 602403

KEGG: hsa:642

STRING: 9606.ENSP00000261714

UniGene: Hs.371914

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.