Human Beta-defensin 104(DEFB104A) ELISA kit

Human Beta-defensin 104(DEFB104A) ELISA kit

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中文名稱：

人β防御素104(DEFB104A)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL006665HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人β防御素104(DEFB104A)酶聯(lián)免疫試劑盒（CSB-EL006665HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的DEFB104A含量。DEFB104A是一個(gè)靶點(diǎn)。其它可能在人體免疫等生理過程中有潛在作用。研究機(jī)制上，可能涉及參與免疫細(xì)胞的調(diào)控、抗菌防御等反應(yīng)，但目前關(guān)于它的詳細(xì)背景及具體研究機(jī)制還需更多研究來(lái)進(jìn)一步明確和完善。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，尤其適用于感染性疾病模型、免疫調(diào)控機(jī)制研究以及抗菌肽相關(guān)的基礎(chǔ)科研，能夠?yàn)轶w外實(shí)驗(yàn)?zāi)Ｐ椭蠨EFB104A的動(dòng)態(tài)變化提供可靠的定量數(shù)據(jù)支持，助力宿主防御機(jī)制及炎癥相關(guān)通路的探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

BD 4 ELISA Kit; BD-4 ELISA Kit; BD4 ELISA Kit; beta 104 ELISA Kit; beta 4 Defensin ELISA Kit; Beta defensin 4 ELISA Kit; Beta-defensin 104 ELISA Kit; Beta-defensin 4 ELISA Kit; D104A_HUMAN ELISA Kit; DEFB-4 ELISA Kit; DEFB104B ELISA Kit; Defensin ELISA Kit; Defensin beta 104 ELISA Kit; Defensin beta 4 precursor ELISA Kit; hBD 4 ELISA Kit; hBD-4 ELISA Kit; hBD4 ELISA Kit
縮寫：

DEFB104A
Uniprot No.：

Q8WTQ1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFB104A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	92
	Range %	87-100
1:4	Average %	85
	Range %	81-93
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of human DEFB104A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-96
EDTA plasma (n=4)	92	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.706	2.655	2.681	2.525
1000	2.136	2.011	2.074	1.918
500	1.346	1.289	1.318	1.162
250	0.764	0.752	0.758	0.602
125	0.367	0.354	0.361	0.205
62.5	0.279	0.287	0.283	0.127
31.25	0.244	0.231	0.238	0.082
0	0.162	0.150	0.156

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
基因功能參考文獻(xiàn)：
1. Beta-defensin 104 displays antimicrobial activity against S. carnosus, E. coli, S. cerevisiae, and P. aeruginosa (strong). PMID: 11481241
2. TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
3. Data suggest an activation of b-defensin-4 induction, in human knee meniscus by the osteoarthritis inflammatory process as a result of an endogenous antibiotic defense mechanism accompanied by an intrinsic effort of tissue remodeling. PMID: 21879330
4. Our results suggest that copy number variation of DEFB4 may not contribute to the pathogenesis of Behcet's disease. PMID: 21385545
5. Analysis of the DEFB4 promoter region shows remarkably high density of sequence variabilities. PMID: 20445567
6. The variations in the genes encoding human beta-defensin-1 and -2 may be associated with the risk of severe acute pancreatitis. PMID: 20720450
7. Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
8. We report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antibacterial peptide hBD4. PMID: 20526896
9. This study demonstrates, the involvement of NOD1 and DEFB4 in direct killing of Helicobacter pylori bacteria by epithelial cells and confirms the importance of NOD1 in host defence mechanisms against cagPAI(+)Helicobacter pylori infection. PMID: 20039881
10. The mRNA level of the beta defensin 4 did not differ significantly between the two subgroups. PMID: 20128731
11. elevated DEFB4 copy number is a risk factor for Crohn's disease PMID: 19809410
12. There is an association of four common DEFB104 haplotypes with the risk of prostate cancer in two independent patient cohorts. PMID: 18515986
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Beta-defensin family
組織特異性：

High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 18115

KEGG: hsa:107984148

STRING: 9606.ENSP00000322191

UniGene: Hs.559507

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.