Human Apolipoprotein C-IV(APOC4) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人載脂蛋白C-IV(APOC4)酶聯(lián)免疫試劑盒（CSB-EL001934HU）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOC4含量。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，科研人員可將其應(yīng)用于脂代謝異常疾病模型構(gòu)建、基因調(diào)控機(jī)制探索或生物樣本中APOC4表達(dá)水平評(píng)估等實(shí)驗(yàn)場(chǎng)景，為分子機(jī)制研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

APO C4 ELISA Kit; Apo CIV ELISA Kit; Apo-CIV ELISA Kit; APOC 4 ELISA Kit; ApoC IV ELISA Kit; ApoC-IV ELISA Kit; APOC4 ELISA Kit; APOC4_HUMAN ELISA Kit; Apolipoprotein C IV ELISA Kit; Apolipoprotein C-IV ELISA Kit; Apolipoprotein C4 ELISA Kit
縮寫(xiě)：

APOC4
Uniprot No.：

P55056
種屬：

Homo sapiens (Human)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-96
1:400	Average %	87
	Range %	84-90
1:800	Average %	95
	Range %	91-99
1:1600	Average %	97
	Range %	92-102

回收率：

The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May participate in lipoprotein metabolism.
基因功能參考文獻(xiàn)：
1. APOC4 rs1132899 polymorphism was associated with an increased risk of premature coronary artery disease in Chinese subjects. PMID: 26129832
2. ApoC-IV overexpression may perturb lipid metabolism leading to lipid accumulation. HCV core protein may modulate ApoC-IV expression through Ku antigen and PPARgamma/RXRalpha complex. PMID: 18809223
亞細(xì)胞定位：

Secreted.
蛋白家族：

Apolipoprotein C4 family
組織特異性：

Expressed by the liver and secreted in plasma.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 611

OMIM: 600745

KEGG: hsa:346

STRING: 9606.ENSP00000468236

UniGene: Hs.731599