Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人2,3-二磷酸甘油酸(2,3-DPG)酶聯免疫試劑盒（CSB-E09861h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的2,3DPG含量。2,3-DPG即2,3-二磷酸甘油酸，是紅細胞糖酵解支路的中間產物。它能調節血紅蛋白對氧氣的親和力，降低其與氧的結合力，促進氧氣釋放。在貧血、高原適應等過程中發揮重要作用。當前對其在相關疾病機制及治療靶點方面有一定研究。試劑盒檢測范圍為0.312 μmol/mL-20 μmol/mL，適用于基礎研究中紅細胞代謝功能評估、缺氧生理機制探索以及血液系統疾病模型的機制分析，例如貧血、高原反應或心血管相關疾病的體外實驗研究，滿足細胞能量代謝、運動生理學或病理狀態下的氧運輸調控等科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

2,3DPG
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 μmol/mL-20 μmol/mL
靈敏度：

0.078 μmol/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	92-100
1:2	Average %	98
Range %	92-103
1:4	Average %	95
Range %	90-99
1:8	Average %	90
Range %	85-96

回收率：

The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	84	80-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

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