Human 14-3-3 protein eta(YWHAH) ELISA kit

Human 14-3-3 protein eta(YWHAH) ELISA kit

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中文名稱：

人14-3-3蛋白eta(YWHAH)酶聯免疫試劑盒
貨號：

CSB-EL026289HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人14-3-3蛋白eta(YWHAH)酶聯免疫試劑盒（CSB-EL026289HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的YWHAH含量。YWHAH 是一種重要靶點。它參與細胞內多種信號通路調控，在細胞的生長、存活、凋亡等過程中發揮關鍵作用。目前圍繞它的研究主要集中于剖析其在疾病發生發展中的作用機制，為開發針對該靶點的治療藥物提供理論依據。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于神經生物學、腫瘤微環境研究及疾病分子機制探索等方向，如阿爾茨海默病腦組織樣本分析、癌癥患者血漿生物標志物檢測或體外模型蛋白表達調控實驗本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

14 3 3 protein eta ELISA Kit; 14-3-3 eta ELISA Kit; 14-3-3 protein eta ELISA Kit; 1433F_HUMAN ELISA Kit; Brain protein 14-3-3; eta isoform ELISA Kit; HGNC:12853 ELISA Kit; Protein AS1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform ELISA Kit; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; YWHA 1 ELISA Kit; YWHA1 ELISA Kit; Ywhah ELISA Kit
縮寫：

YWHAH
Uniprot No.：

Q04917
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	97
	Range %	93-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	102
	Range %	86-105

回收率：

The recovery of human YWHAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	88	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.632	2.756	2.694	2.592
20	2.224	2.376	2.300	2.198
10	1.646	1.748	1.697	1.595
5	0.934	0.950	0.942	0.840
2.5	0.524	0.508	0.516	0.414
1.25	0.356	0.362	0.359	0.257
0.625	0.242	0.245	0.244	0.142
0	0.103	0.101	0.102

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

■ 常見問題解答

I am interested in human 14-3-3eta ELISA kit
What kind of positive control was used to check the kit?
I am planning to use primary cell culture supernatant
I have an experience with other companies ELISA plate. That company used as positive control recombinant 14-3-3eta derived from YEAST. I tried as control recombinant 14-3-3eta derived from E.Coli at same concentration and got negative data. That is why I am searching for other ELISA kit and wondering if CUSABIO had checked ELISA kit with serum, cells lysate also, not only recombinant protein.
One more question. What is the binding site of the anti-14-3-3eta antibody used at this ELISA kit? Thank you!
Excuse me, this is the product I am interested in https://www.cusabio.com/ELISA-Kit/Human-14-3-3-protein-etaYWHAH-ELISA-kit-112977.html

Thanks for your inquir!Our elisa kits are provided with the standard in it.The standard performs as a quality control and it comes as a complete system together with other reagents in the kit.
Regarding this elisa kit,the standard is express with CHO and 2 antibodies are used in it,mouse monoclonal antibody and goat polyclonal antibody.While the specific recognition site is not clear.
We tested and validated this kit on serum and plasma samples and this kit is usually used to detect serum and plasma samples.For cells lysate sample,we suggest the customer need do pre-testing first.If the customer uses the recombinant protein of the same structure with the natural protein,then it is ok to use it to detect the recombinant protein.If the customer must detect the recombinant protein,he/she had better to use the the recombinant protein expressed by eukaryote.While for the specific situation,pls refer to the customer's actual testing results.Natural protein is for sure the best.
As to the elisa kits from other brands,we did not ever analyzed and it is hard to comment.For the mentioned negative results,we suppose it happens.Pls do the specific analysis accordingly.

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功能：

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
基因功能參考文獻：
1. Results show that YWHAZ amplification indicates a better survival trend in bladder cancer. Its knockdown promotes both in vitro and in vivo tumorigenesis in bladder cancer and may be a novel biomarker for bladder cancer. PMID: 29512774
2. The impact of AKT1 on glucocorticoid receptor (GR)-induced transcriptional activity in cooperation with phospho-serine/threonine-binding protein 14-3-3, was examined. PMID: 27717743
3. 14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis. PMID: 27256400
4. Extracellular 14-3-3eta activates key signalling cascades and induces factors associated with the pathogenesis of rheumatoid arthritis PMID: 24751211
5. This study identified YWHAH significantly associated loci with a biologically plausible role in schizophrenia. PMID: 24507884
6. 14-3-3eta may be required for mitotic progression and may be considered as a novel anti-cancer strategy in combination with microtubule inhibitors. PMID: 22562251
7. This is the first genetic association study of YWHAH with sporadic Creutzfeldt-Jakob disease populations. PMID: 21739144
8. relocation of p33ING1b from the nucleus to the cytoplasm, where the protein is tethered by 14-3-3eta, participates in tumorigenesis and progression in HNSCC PMID: 21432775
9. The 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
10. phosphorylation of 14-3-3 binding site of myeloid leukemia factor 1 by MADM PMID: 12176995
11. Data demonstrate that 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, but there was no specific staining for the 14-3-3 eta subunit. PMID: 12480176
12. gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein PMID: 16545136
13. The association of YWHAH with bipolar disorder in a large sample, consisting of 1211 subjects from 318 nuclear families including 554 affected offspring, was investigated. PMID: 19160447
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蛋白家族：

14-3-3 family
組織特異性：

Expressed mainly in the brain and present in other tissues albeit at lower levels.
數據庫鏈接：

HGNC: 12853

OMIM: 113508

KEGG: hsa:7533

STRING: 9606.ENSP00000248975

UniGene: Hs.226755

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.