Horse growth hormone,GH ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

馬生長激素(GH)酶聯免疫試劑盒（CSB-E11242Eq）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 為生長激素 1 基因，其表達產物生長激素與人體生長發育密切相關。研究機制方面，它主要通過與受體結合，激活多種信號通路，促進細胞增殖、分化和蛋白質合成。異常的 GH1 表達或功能可能引發如巨人癥、侏儒癥等生長異常疾病。試劑盒檢測范圍為25 ng/mL-400 ng/mL，適用于科研領域探究馬匹生長調控、營養代謝、繁殖性能及疾病模型中的GH動態變化。適用于實驗室對馬科動物生理病理機制研究、激素相關藥物開發評估以及畜牧領域GH水平監測等科研需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
縮寫：

GH1
Uniprot No.：

P01245
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 ng/mL-400 ng/mL
靈敏度：

25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	87
	Range %	84-90
1:4	Average %	96
	Range %	90-102
1:8	Average %	90
	Range %	85-96

回收率：

The recovery of horse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	96	91-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能參考文獻：
1. Plasma levels of growth hormone (as well as glucose, insulin, and leptin) are highly correlated with the duration of winter anovulatory phase. PMID: 21958120
亞細胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數據庫鏈接：

KEGG: ecb:100034180

STRING: 9796.ENSECAP00000007142

UniGene: Eca.12604