Horse Tumor necrosis factor α,TNF-α ELISA Kit

Horse Tumor necrosis factor α,TNF-α ELISA Kit

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文獻引用 (3) 說明書下載

中文名稱：

馬腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E14031Hs
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

馬腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E14031Hs）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種多功能促炎細胞因子，主要由巨噬細胞分泌，與腫瘤壞死因子受體結合發揮功能。其在細胞凋亡、炎癥和免疫反應中起重要作用，與多種疾病如腫瘤、炎癥和自身免疫性疾病相關。研究TNF機制有助于開發針對相關疾病的治療策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研場景下對馬屬動物炎癥相關模型的機制研究、藥物干預效果評價或基礎免疫學分析，例如通過檢測血清或組織中的TNF-α濃度動態，揭示炎癥相關疾病的分子調控網絡。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
縮寫：

TNF
Uniprot No.：

P29553
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-97
1:2	Average %	92
	Range %	84-99
1:4	Average %	95
	Range %	92-103
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	83-98
EDTA plasma (n=4)	94	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.601	2.548	2.575	2.409
500	2.248	2.211	2.230	2.064
250	1.631	1.616	1.624	1.458
125	0.905	0.925	0.915	0.749
62.5	0.475	0.483	0.479	0.313
31.2	0.312	0.321	0.317	0.151
15.6	0.255	0.268	0.262	0.096
0	0.164	0.167	0.166

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia IE Helal, HA Al-Abbadi, MA Hashem, HMA Abdelrazek,Animals,2024
Expression of Vitamin D Receptor Gene (VDR) and Associated Cytokine Response in Arabian Horses with Chronic Inflammatory Airway Disease H Haddadi,/,2025
Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells Abdelhamid L.et al,Res Vet Sci,2017

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with equine metabolic syndrome was enhanced while TNF-alpha levels of both groups were comparable. PMID: 25269712
2. Data indicate that tumor necrosis factor-alpha production is closely related to ovarian steroid actions. PMID: 24642167
3. These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation. PMID: 23941776
4. These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function. PMID: 22186103
5. These results suggest that basal oxidative stress markers, circulating cytokines and anti-inflammatory neuroendocrine hormones appear to correlate with endurance performance in horses. PMID: 21058993
6. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392
7. The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: ecb:100033834

STRING: 9796.ENSECAP00000001460

UniGene: Eca.13019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.