Horse Progesterone(PROG) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

馬孕激素/孕酮(PROG)酶聯免疫試劑盒（CSB-E13183Hs）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PROG含量。試劑盒檢測范圍為0.25 ng/mL-100 ng/mL，靈敏度為0.25 ng/mL。本試劑盒采用高特異性抗體，可精準識別樣本中的孕酮分子，適用于科研場景下的馬科動物生殖生理研究、妊娠狀態追蹤、繁殖效率評估及激素代謝分析。實驗流程經優化設計，配套標準品及反應體系完備，可在常規實驗室條件下快速完成檢測，為畜牧業繁殖優化、野生動物保護等領域的激素水平研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PROG
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma
檢測范圍：

0.25 ng/mL-100 ng/mL
靈敏度：

0.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-107
1:2	Average %	89
	Range %	83-99
1:4	Average %	95
	Range %	85-103
1:8	Average %	90
	Range %	86-102

回收率：

The recovery of horse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-106
EDTA plasma (n=4)	95	82-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average
100	0.162	0.158	0.160
18.75	0.320	0.332	0.326
3.125	0.542	0.563	0.553
0.875	0.971	0.944	0.958
0.25	1.503	1.485	1.494
0	2.192	2.127	2.160

Evaluation of circulating miRNAs in mares approaching parturition M Kikuchi, H Murase, K Urata, T Ishige, T Tozaki,Domestic Animal Endocrinology,2024

最新研究進展：

PROG是一種激素，可通過作用于孕酮受體來調節生殖系統、乳房和神經系統的功能。近年來，PROG被廣泛研究，尤其在抗腫瘤治療和神經保護方面的應用潛力備受關注。多項研究表明，PROG可以通過多種機制發揮抗腫瘤作用，如調節細胞周期、促進細胞凋亡、抑制血管生成等。同時，PROG也具有保護神經元、促進神經元再生的作用，可以減輕多種神經退行性疾病的癥狀。這些研究為PROG的臨床應用提供了新思路和方向。