Horse Gelsolin(GSN) ELISA kit

Horse Gelsolin(GSN) ELISA kit

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文獻引用 (1) 說明書下載

中文名稱：

馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒
貨號：

CSB-EL009965HO
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒（CSB-EL009965HO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GSN含量。GSN 是一個靶點。它在細胞中具有重要作用，與細胞的運動、形態(tài)維持等相關(guān)。研究機制方面，其可能參與調(diào)節(jié)細胞骨架的動態(tài)變化。通過對它的深入研究，有望揭示疾病發(fā)生發(fā)展機制，為開發(fā)新的治療方法和藥物提供方向。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，該產(chǎn)品可為馬屬動物細胞遷移機制、炎癥相關(guān)疾病模型或肌肉組織再生等基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)支持，適用于實驗室條件下對生物樣本中GSN蛋白表達水平的動態(tài)監(jiān)測及比較分析，為探索其分子功能及調(diào)控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
縮寫：

GSN
Uniprot No.：

Q28372
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

6.25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	94-105
1:2000	Average %	89
	Range %	82-94
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	95
	Range %	91-99

回收率：

The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	91	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
800	0.234	0.228	0.231
400	0.462	0.453	0.458
200	0.710	0.684	0.697
100	1.028	1.001	1.015
50	1.276	1.245	1.261
25	1.584	1.532	1.558
12.5	1.683	1.623	1.653
0	1.827	1.789	1.808

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Synovial fluid alpha‐2‐macroglobulin, gelsolin and lubricin distinguish between osteoarthritic and healthy equine joints EJ Secor, SJ Womack, MP Ysebaert,Equine Veterinary Journal,2025

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能參考文獻：
1. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
亞細胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Villin/gelsolin family
數(shù)據(jù)庫鏈接：

KEGG: ecb:100034186

UniGene: Eca.2517

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.