Horse Clusterin(CLU) ELISA kit

說明書下載

中文名稱：

馬凝聚素(CLU)酶聯免疫試劑盒
貨號：

CSB-EL005595HO
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

馬凝聚素(CLU)酶聯免疫試劑盒（CSB-EL005595HO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CLU含量。CLU（Clusterin）是一種存在于多種細胞組分中的多功能蛋白，最早發現于山羊精液中，參與細胞間黏附。研究顯示CLU與神經退行性疾病、免疫疾病、衰老和癌癥等多種病理狀態相關。CLU具有不同的蛋白產物，如分泌型（sCLU）和核型（nCLU），在細胞定位和生物學功能上有所不同。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，本產品適用于科研領域對CLU蛋白表達水平的精準分析，為研究CLU在心血管疾病、神經退行性病變及腫瘤微環境中的分子機制提供了可靠工具，也可用于評估實驗動物模型或體外培養體系中CLU的干預調控效果。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CLU ELISA Kit; Clusterin [Cleaved into: Clusterin beta chain; Clusterin alpha chain] ELISA Kit
縮寫：

CLU
Uniprot No.：

Q29482
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	95-106
1:2000	Average %	90
	Range %	85-94
1:4000	Average %	98
	Range %	92-102
1:8000	Average %	100
	Range %	94-104

回收率：

The recovery of horse CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-96
EDTA plasma (n=4)	102	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1000	0.171	0.167	0.169
500	0.277	0.264	0.271
250	0.498	0.473	0.486
125	0.811	0.819	0.815
62.5	1.349	1.330	1.340
31.25	1.906	1.878	1.892
15.6	2.412	2.335	2.374
0	2.950	2.876	2.913

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Following stress, promotes apoptosis. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury.
基因功能參考文獻：
1. The secretion of the potential zonal marker clusterin by zonal articular chondrocytes in osteoarthritic and healthy articular cartilage, was characterized. PMID: 19814590
亞細胞定位：

Secreted. Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Mitochondrion. Mitochondrion membrane. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
蛋白家族：

Clusterin family
數據庫鏈接：

KEGG: ecb:100034172

STRING: 9796.ENSECAP00000005450

UniGene: Eca.18000

Horse Clusterin(CLU) ELISA kit

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