Hamster C-reactive protein(CRP) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

倉鼠C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-EL005991HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CRP含量。CRP（C反應(yīng)蛋白）是一種急性時相反應(yīng)蛋白，在機(jī)體發(fā)生感染、炎癥、組織損傷時，血漿中CRP水平會急劇升高。其研究機(jī)制主要集中在參與炎癥反應(yīng)調(diào)節(jié)，可激活補(bǔ)體系統(tǒng)、調(diào)節(jié)免疫細(xì)胞功能等，常作為炎癥和疾病活動性的重要監(jiān)測指標(biāo)。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于倉鼠模型中的炎癥機(jī)制研究、藥物干預(yù)效果評價或疾病相關(guān)生物標(biāo)志物探索等科研場景，為實(shí)驗動物模型的炎癥狀態(tài)監(jiān)測提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CRP ELISA kit; PTX1C-reactive protein ELISA kit
縮寫：

CRP
Uniprot No.：

P49262
種屬：

Mesocricetus auratus (Golden hamster)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	85-96
1:10	Average %	87
	Range %	82-92
1:20	Average %	96
	Range %	89-103
1:40	Average %	98
	Range %	94-104

回收率：

The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-93
EDTA plasma (n=4)	96	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Pentraxin family
組織特異性：

Found in plasma.