Guinea pig interleukin 5,IL-5 ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠白介素5(IL-5)酶聯免疫試劑盒（CSB-E12064Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL5含量。IL5是重要的治療靶點。它主要由活化的T淋巴細胞產生，能促進嗜酸性粒細胞的生長、分化和存活。針對IL5的研究主要圍繞阻斷其與受體結合，以減少嗜酸性粒細胞數量，進而治療哮喘、嗜酸性粒細胞增多癥等嗜酸性粒細胞相關疾病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于豚鼠疾病模型的免疫機制研究，例如哮喘、寄生蟲感染等實驗中的炎癥因子動態監測，或用于評估免疫調節藥物對IL - 5通路的影響。組織勻漿樣本可用于分析肺、骨髓等靶器官的局部IL - 5表達水平，為科研人員提供穩定可靠的定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
縮寫：

IL5
Uniprot No.：

O08987
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	103
	Range %	98-108
1:8	Average %	100
	Range %	91-105

回收率：

The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	92	84-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
亞細胞定位：

Secreted.
蛋白家族：

IL-5 family
數據庫鏈接：

STRING: 10141.ENSCPOP00000004355