Guinea pig interleukin 4,IL-4 ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠白介素4(IL-4)酶聯免疫試劑盒（CSB-E10126Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-4含量。IL-4是一種多效細胞因子，參與免疫應答的調節。主要由肥大細胞、T細胞等分泌，通過IL-4 RA受體招募γc鏈，形成I型信號復合物，介導細胞生長、抗凋亡、基因激活和分化。此外，IL-4還參與調節T細胞分化、免疫球蛋白類轉換等，在免疫和炎癥過程中發揮重要作用。試劑盒檢測范圍為3.12 pg/mL-200pg/mL，適用于豚鼠模型的免疫機制研究，特別是在過敏性疾病、寄生蟲感染、哮喘模型等Th2型免疫反應相關科研領域，為評估實驗動物體內IL - 4動態變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-4
Uniprot No.：

G0WM66
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-105
1:2	Average %	96
	Range %	92-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.