Guinea pig interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豚鼠白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E12914Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-2含量。IL-2是一種重要的細(xì)胞因子，在免疫系統(tǒng)中扮演關(guān)鍵角色。它能促進(jìn)T細(xì)胞、B細(xì)胞的增殖分化，增強(qiáng)自然殺傷細(xì)胞活性等。科研人員深入研究其與免疫調(diào)節(jié)相關(guān)的信號傳導(dǎo)通路等機(jī)制，為開發(fā)免疫治療藥物、攻克相關(guān)疾病提供了理論基礎(chǔ)。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域探究豚鼠感染模型中的免疫激活狀態(tài)、評估疫苗或免疫調(diào)節(jié)劑的干預(yù)效果，以及炎癥性疾病機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-2
Uniprot No.：

H0VBY4
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-108
1:2	Average %	94
	Range %	91-98
1:4	Average %	103
	Range %	97-108
1:8	Average %	88
	Range %	80-96

回收率：

The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-100
EDTA plasma (n=4)	104	96-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.