Guinea pig insulin (INS) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠胰島素(INS)酶聯免疫試劑盒（CSB-EL011742GU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INS含量。INS 即胰島素基因，其編碼的胰島素是調節血糖的關鍵激素。在研究機制上，主要聚焦于其表達調控、胰島素信號通路等。深入研究有助于理解糖尿病等代謝疾病發病機制，也為開發相關治療藥物，如胰島素類似物等提供理論基礎。試劑盒檢測范圍為12 μIU/mL-210 μIU/mL，適用于豚鼠模型中胰島素相關科研分析；在糖尿病機制研究、代謝性疾病模型構建及藥物干預效果評價等領域具有重要應用價值；可為胰腺功能研究、胰島素分泌調控實驗提供精準數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
縮寫：

INS
Uniprot No.：

P01329
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12 μIU/mL-210 μIU/mL
靈敏度：

7.5 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-97
1:2	Average %	96
	Range %	90-106
1:4	Average %	95
	Range %	86-104
1:8	Average %	90
	Range %	87-94

回收率：

The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-101
EDTA plasma (n=4)	95	89-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
亞細胞定位：

Secreted.
蛋白家族：

Insulin family
數據庫鏈接：

STRING: 10141.ENSCPOP00000019016