Guinea pig Interleukin-1 beta(IL1B) ELISA kit

Guinea pig Interleukin-1 beta(IL1B) ELISA kit

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中文名稱：

豚鼠白介素1β(IL1B)酶聯(lián)免疫試劑盒
貨號：

CSB-EL011614GU
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豚鼠白介素1β(IL1B)酶聯(lián)免疫試劑盒（CSB-EL011614GU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B，即白介素-1β，是一種重要的促炎細(xì)胞因子，主要由單核細(xì)胞、巨噬細(xì)胞和樹突狀細(xì)胞產(chǎn)生。它在炎癥和免疫反應(yīng)中起關(guān)鍵作用，通過激活I(lǐng)L-1受體和下游信號通路，調(diào)節(jié)細(xì)胞增殖、分化和凋亡。IL1B的產(chǎn)生和活性受多種炎癥刺激調(diào)控，如微生物產(chǎn)物和內(nèi)源性危險相關(guān)分子。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于豚鼠模型的基礎(chǔ)科研領(lǐng)域，如炎癥機制研究、免疫相關(guān)疾病動物模型分析、藥物或化合物抗炎效果評估等場景，為探索豚鼠在病原體感染、創(chuàng)傷修復(fù)或免疫干預(yù)實驗中IL1β的調(diào)控機制提供可靠工具，滿足實驗室對炎癥標(biāo)志物的精準(zhǔn)定量需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
縮寫：

IL1B
Uniprot No.：

Q9WVG1
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	97
	Range %	85-100
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of guinea pig IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	88-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.400	2.457	2.429	2.295
500	1.955	1.963	1.959	1.825
250	1.324	1.351	1.338	1.204
125	0.838	0.829	0.834	0.700
62.5	0.529	0.556	0.543	0.409
31.2	0.334	0.341	0.338	0.204
15.6	0.232	0.238	0.235	0.101
0	0.134	0.133	0.134

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Tobacco Seed-Based Oral Vaccination against Verocytotoxic O138 Escherichia coli as Alternative Approach to Antibiotics in Weaned Piglets L Rossi,Antibiotics,2023
Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide Y Li,Journal of Animal Science,2022
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota Qingwei Meng, et al,Food & Function,2019

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亞細(xì)胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數(shù)據(jù)庫鏈接：

STRING: 10141.ENSCPOP00000001784

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.