Guinea pig Interferon γ,IFN-γ ELISA Kit

Guinea pig Interferon γ,IFN-γ ELISA Kit

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中文名稱：

豚鼠γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
貨號：

CSB-E06763Gu
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豚鼠γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E06763Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFN-γ含量。IFN-γ即γ-干擾素，是具有多種生物學(xué)活性的細(xì)胞因子。背景上，它在免疫調(diào)節(jié)等機(jī)體生理功能中扮演重要角色。研究機(jī)制方面，其可激活巨噬細(xì)胞、增強(qiáng)NK細(xì)胞活性等，在抗感染、抗腫瘤及免疫相關(guān)疾病研究中受廣泛關(guān)注。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，尤其適用于豚鼠模型的感染性疾病機(jī)制探索和疫苗開發(fā)等科研領(lǐng)域；適用于評估免疫激活狀態(tài)或炎癥反應(yīng)強(qiáng)度；該產(chǎn)品廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、獸醫(yī)學(xué)研究中宿主免疫應(yīng)答分析、病原體感染模型建立以及生物制劑效果評價(jià)等非診斷性科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IFN-gamma，interferon gamma
縮寫：

IFN-γ
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.7 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	88-108
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	90	86-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.075	2.051	2.063	1.965
600	1.465	1.441	1.453	1.355
300	1.069	1.045	1.057	0.959
150	0.655	0.622	0.639	0.541
75	0.400	0.387	0.394	0.296
37.5	0.320	0.295	0.308	0.210
18.75	0.205	0.223	0.214	0.116
0	0.101	0.095	0.098

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Characterization of immune development of fetal and early-life of minipigs J Cho,International immunopharmacology,2023
Isoleucine attenuates infection induced by E. coli challenge through modulation of intestinal endogenous antimicrobial peptide expression and inhibition of the increase of plasma endotoxin and IL-6 in weaned pigs Man Ren,Food & Function,2019
Dietary xylo-oligosaccharide improves intestinal function in weaned piglets Jie Yin, et al,Food & Function,2019
Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
Augmentation of DTH reaction of mycobacterial antigenic cocktail using synthetic mycobacterial 19-kDa lipoprotein as a TLR-stimulant Mohamed AM, et al,Journal of immunoassay & immunochemistry,2018
The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection Filipovic A.et al,PLoS One,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.