Goat follicle-stimulating hormone,FSH ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊促卵泡素(FSH)酶聯(lián)免疫試劑盒（CSB-E13277G）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FSHB含量。FSHB（促卵泡激素β亞基）是一種由人類基因FSHB編碼的蛋白質，位于垂體前葉，對生殖功能至關重要。FSHB與FSHα亞基結合，形成促卵泡激素（FSH），調節(jié)性腺活動，包括卵泡發(fā)育和精子生成。研究顯示，F(xiàn)SHB基因突變可能導致生殖能力下降。試劑盒檢測范圍為20 mIU/mL-800 mIU/mL，適用于科研領域中對山羊繁殖生理機制、生殖疾病模型構建、畜牧業(yè)繁殖力優(yōu)化等研究，例如發(fā)情周期動態(tài)監(jiān)測、卵巢功能評估、激素干預效果分析等場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
縮寫：

FSHB
Uniprot No.：

Q8HY83
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma
檢測范圍：

20 mIU/mL-800 mIU/mL
靈敏度：

20 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-108
1:2	Average %	88
	Range %	82-98
1:4	Average %	95
	Range %	85-105
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of goat FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	92	87-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫鏈接：

UniGene: Chi.38676