Goat adiponectin(ADP)ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

山羊脂聯(lián)素(ADP)酶聯(lián)免疫試劑盒（CSB-E15981G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADP含量。ADP即二磷酸腺苷，是生物體內(nèi)能量代謝的關(guān)鍵分子。在細(xì)胞代謝中，它參與能量的儲存和釋放。研究機制方面，ADP可與相關(guān)受體結(jié)合，調(diào)節(jié)血小板聚集等生理過程，還在信號傳導(dǎo)等通路中發(fā)揮作用，成為藥物研發(fā)等領(lǐng)域的重要靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，本試劑盒適用于肥胖、2型糖尿病、心血管疾病等代謝相關(guān)機制的基礎(chǔ)研究，可支持動物模型或體外實驗中脂聯(lián)素表達水平的動態(tài)監(jiān)測，為探索能量代謝調(diào)控、脂肪因子互作等科研方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ADP
Uniprot No.：

U6A087
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	89-100
1:2000	Average %	92
	Range %	87-96
1:4000	Average %	99
	Range %	93-104
1:8000	Average %	104
	Range %	97-109

回收率：

The recovery of goat ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-97
EDTA plasma (n=4)	94	87-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.