Goat Tri-iodothyronine(T3) ELISA KIT

說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

山羊三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒（CSB-E13385G）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的T3含量。試劑盒檢測(cè)范圍為0.5 ng/mL-8 ng/mL，靈敏度為0.5 ng/mL。適用于科研領(lǐng)域如畜牧動(dòng)物生理研究、飼料營(yíng)養(yǎng)干預(yù)實(shí)驗(yàn)、甲狀腺相關(guān)疾病機(jī)制探索及藥物代謝評(píng)估等方向，有助于研究山羊內(nèi)分泌功能、代謝疾病模型或環(huán)境因素對(duì)甲狀腺軸的影響本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

T3
種屬：

Capra hircus (Goat)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.5 ng/mL-8 ng/mL
靈敏度：

0.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-103
1:2	Average %	95
	Range %	85-102
1:4	Average %	93
	Range %	90-97
1:8	Average %	94
	Range %	88-99

回收率：

The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-102
EDTA plasma (n=4)	96	92-100

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

近期研究表明，T3在許多重要的生理過(guò)程中扮演著關(guān)鍵角色，如代謝調(diào)節(jié)、心臟功能、神經(jīng)發(fā)育和免疫系統(tǒng)等。此外，一些研究還發(fā)現(xiàn)，T3可能在腫瘤生長(zhǎng)和轉(zhuǎn)移中發(fā)揮一定作用，值得深入探究。