Goat Interleukin 4,IL-4 ELISA Kit

Goat Interleukin 4,IL-4 ELISA Kit

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中文名稱：

山羊白介素4(IL-4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09808g
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

山羊白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E09808g）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL4含量。IL4是重要的細(xì)胞因子。其背景上，它在免疫系統(tǒng)中起關(guān)鍵作用，參與免疫反應(yīng)調(diào)節(jié)。研究機(jī)制方面，IL4能作用于多種免疫細(xì)胞，如T、B淋巴細(xì)胞等，可調(diào)節(jié)細(xì)胞的增殖、分化，還參與Th2細(xì)胞相關(guān)免疫應(yīng)答過(guò)程，與過(guò)敏等疾病病理機(jī)制關(guān)聯(lián)密切。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，可應(yīng)用于動(dòng)物免疫學(xué)基礎(chǔ)研究，如寄生蟲感染模型中的Th2免疫應(yīng)答評(píng)估、過(guò)敏性疾病機(jī)制探究或疫苗效價(jià)研究中的免疫指標(biāo)分析，為科研人員解析山羊免疫調(diào)控網(wǎng)絡(luò)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

P79155
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of goat IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	96	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.775	2.799	2.787	2.645
500	2.109	1.998	2.054	1.912
250	1.371	1.387	1.379	1.237
125	0.791	0.786	0.789	0.647
62.5	0.505	0.541	0.523	0.381
31.2	0.357	0.368	0.363	0.221
15.6	0.278	0.298	0.288	0.146
0	0.141	0.143	0.142

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Construction of a Triple-Gene Deletion Mutant of Orf Virus and Evaluation of Its Safety, Immunogenicity and Protective Efficacy Z Shen,Vaccines,2023
Peste des Petits Ruminants Virus-Like Particles Induce a Potent Humoral and Cellular Immune Response in Goats Yan F, et al,Viruses,2019

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: chx:100860814

UniGene: Chi.38713

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.