Goat Interleukin 2(IL-2) ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

山羊白介素2(IL-2)酶聯免疫試劑盒（CSB-E12965G）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的細胞因子，由T細胞分泌。其背景在于對免疫系統有關鍵調節作用。研究機制上，IL2能與特異性受體結合，激活T細胞和NK細胞等免疫細胞的增殖與分化，增強機體免疫功能，在腫瘤免疫治療等方面是研究熱點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領域中對山羊免疫相關疾病的機制探索、炎癥模型分析或疫苗開發中細胞免疫應答的定量評估；為獸醫研究、比較免疫學及畜牧領域中的免疫調控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
縮寫：

IL2
Uniprot No.：

P36835
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	94-107
1:8	Average %	94
	Range %	87-98

回收率：

The recovery of goat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-102
EDTA plasma (n=4)	93	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
亞細胞定位：

Secreted.
蛋白家族：

IL-2 family
數據庫鏈接：

UniGene: Chi.38711