Goat Interleukin 1β(IL-1β) ELISA Kit

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中文名稱：

山羊白介素1β(IL-1β)酶聯免疫試劑盒
貨號：

CSB-E16568G
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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山羊白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E16568G）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B即白介素-1β，是重要炎癥介質。它在炎癥反應、免疫調節等生理病理過程中起關鍵作用。其研究機制主要圍繞它與受體結合后激活下游信號通路，引發炎癥級聯反應，目前針對它的干預研究有望為炎癥相關疾病治療提供新思路。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于動物模型研究、炎癥機制探索、免疫調控實驗等科研領域，能夠為病原體感染評估、炎癥因子網絡分析、免疫干預效果驗證等基礎研究提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
縮寫：

IL-1β
Uniprot No.：

P79162
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-94

1:2

Average %

Range %

82-97

1:4

Average %

Range %

85-100

1:8

Average %

Range %

90-103

回收率：

The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-98
EDTA plasma (n=4)	99	93-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.112	2.052	2.082	1.951
100	1.576	1.654	1.615	1.484
50	1.039	0.984	1.012	0.881
25	0.587	0.549	0.568	0.437
12.5	0.384	0.397	0.391	0.260
6.25	0.292	0.269	0.281	0.150
3.12	0.208	0.199	0.204	0.073
0	0.127	0.134	0.131

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Dietary Tea Tree (Melaleuca alternifolia) Oil Supplementation Improves Growth Performance, Cecal Microflora, Immunity, and Antioxidant Capacity of Partridge Shank Chickens X Lv,Food Science & Nutrition,2022
Investigation of acute-phase proteins and cytokines response in goats with contagious caprine pleuropneumonia with special reference to their diagnostic accuracy. W El-Deeb,PeerJ,2020
Rhubarb supplementation promotes intestinal mucosal innate immune homeostasis through modulating intestinal epithelial microbiota in goat kids Jinzhen Jiao.et al,J Agric Food Chem,2018
Cytokines (IL 1β and IL 6) Responses in Non-Pregnant Does Infected with Corynebacterium pseudotuberculosis Following Intradermal Route of Infection in Chronic State Jesse F F A.et al,International Journal of Livestock Research,2016

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功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

UniGene: Chi.15482

Goat Interleukin 1β(IL-1β) ELISA Kit

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