Goat Immunoglobulin M(IgM) ELISA Kit

文獻(xiàn)引用 (1) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

山羊免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒（CSB-E13134G）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是一種免疫球蛋白，是機(jī)體初次體液免疫應(yīng)答中最早產(chǎn)生的抗體。在人體免疫系統(tǒng)中發(fā)揮著重要作用，能識別并結(jié)合病原體。研究機(jī)制上，主要圍繞其與抗原的結(jié)合特性、激活補(bǔ)體系統(tǒng)等方面展開，以探索其在疾病診斷與治療中的應(yīng)用。試劑盒檢測范圍為24.4 ng/mL-6250 ng/mL，評估疫苗接種后的抗體應(yīng)答水平、追蹤感染性疾病進(jìn)程中的免疫動態(tài)，或探究營養(yǎng)干預(yù)對免疫功能的調(diào)控機(jī)制，為畜牧科研、獸醫(yī)學(xué)研究及動物模型構(gòu)建提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgM
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma
檢測范圍：

24.4 ng/mL-6250 ng/mL
靈敏度：

24.4 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	94
	Range %	89-103
1:10000	Average %	89
	Range %	81-99
1:20000	Average %	97
	Range %	89-105
1:40000	Average %	95
	Range %	83-98

回收率：

The recovery of goat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-105
EDTA plasma (n=4)	95	88-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.