Fish Interleukin 1β (IL- 1β) ELISA Kit

文獻引用 (14) 說明書下載

中文名稱：

魚白介素1β(IL-1β)酶聯免疫試劑盒
貨號：

CSB-E13259Fh
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

魚白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E13259Fh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B是重要的炎癥細胞因子，在多種炎癥性疾病及免疫反應中起關鍵作用。研究機制上，它參與激活免疫細胞、調節炎癥信號通路。圍繞IL1B的研究聚焦于其在疾病發生發展中的作用，為開發抗炎、免疫調節藥物提供靶點。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本產品廣泛應用于水產動物免疫學研究、魚類病原感染模型構建、疫苗效力評估以及水產養殖環境脅迫對機體免疫功能影響等科研場景，為深入解析魚類先天免疫機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

il1bInterleukin-1 beta ELISA Kit
縮寫：

IL-1β
Uniprot No.：

Q9YGD3
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	94
	Range %	90-99
1:4	Average %	104
	Range %	97-108
1:8	Average %	99
	Range %	94-106

回收率：

The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	89	82-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.989	2.778	2.884	2.776
100	2.467	2.432	2.450	2.342
50	1.774	1.866	1.820	1.712
25	0.956	0.932	0.944	0.836
12.5	0.436	0.456	0.446	0.338
6.25	0.258	0.270	0.264	0.156
3.12	0.156	0.155	0.156	0.048
0	0.105	0.111	0.108

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
Effects of mandarin peel powder on growth, biochemical, immune, and intestinal health in Oreochromis niloticus at suboptimal temperatures RM Reda, MIA El-Rahim, DA Elkerdawy,BMC Veterinary Research,2024
Effects of Low Concentration of Glyphosate‐Based Herbicide on Genotoxic, Oxidative, Inflammatory, and Behavioral Meters in Danio rerio (Teleostei and Cyprinidae) E Bortolon Ribas, G Colombo Dal-Pont,Biochemistry Research International,2024
Embryonic dexamethasone exposure exacerbates hepatic steatosis and APAP-mediated liver injury in zebrafish C Chang,Ecotoxicology and environmental safety,2024
Comparison of gut toxicity and microbiome effects in zebrafish exposed to polypropylene microplastics: interesting effects of UV-weathering on microbiome JS Choi,Journal of hazardous materials,2024
Growth, Nutrient Deposition, Plasma Metabolites, and Innate Immunity are Associated with Feeding Rate in Juvenile Starry Flounder (Platichthys Stellatus) JH Cho,/,/
Aeromonas veronii detection in Egyptian fish farms with summer tilapia mortality outbreaks and the role of formic acid in limiting its spread RM Reda,Philippine Journal of Science,2023
Vaccination with bacterial ghosts of Streptococcus iniae and Lactococcus garvieae originated from outbreak of marine fish streptococcosis, induce potential protection against the disease in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) MMA Hussein,Fish & shellfish immunology,2023
Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
Functional and Structural Characterization of Zebrafish ASC Yajuan Li.et al,FEBS J,2018
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016

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功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
基因功能參考文獻：
1. Major changes due to graded levels of olive leaf extract in the diets were observed in blood biochemical parameters. TNFalpha, IL1-beta and IL-8 gene expressions were significanlty up-regulated in OLE 0.1% group compared with others. PMID: 29733961
2. The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated. PMID: 21184658
亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.
蛋白家族：

IL-1 family
組織特異性：

All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.
數據庫鏈接：

UniGene: Omy.11696

Fish Interleukin 1β (IL- 1β) ELISA Kit

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