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中文名稱：魚熱休克蛋白70(HSP-70)酶聯免疫試劑盒
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貨號：CSB-E16327Fh
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規格：96T/48T
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價格：￥3800/￥2500
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其他：
產品詳情
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產品描述：魚熱休克蛋白70(HSP-70)酶聯免疫試劑盒（CSB-E16327Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSP70含量。HSP70是重要的熱休克蛋白，在細胞應激反應中起關鍵作用，廣泛參與蛋白質折疊、轉運和降解等過程。研究發現其異常表達與腫瘤、神經退行性疾病等多種疾病相關，抑制或激活HSP70功能正成為潛在治療策略與研究熱點。試劑盒檢測范圍為18.75 pg/mL-300 pg/mL，該產品為科研人員提供可靠工具，可應用于水產養殖健康監測、環境壓力評估、魚類生理機制研究等領域，為水生生物應激反應模型的建立及生態毒理學實驗提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
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縮寫：HSP-70
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：18.75 pg/mL-300 pg/mL
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靈敏度：18.75 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 98 Range % 92-103 1:4 Average % 82 Range % 80-85 1:8 Average % 91 Range % 83-99 -
回收率：
The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 94 91-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 300 0.153 0.144 0.149 150 0.255 0.242 0.249 75 0.519 0.533 0.526 37.5 1.103 1.134 1.119 18.75 2.363 2.270 2.317 -
數據處理：
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貨期：3-5 working days
引用文獻
- Transcriptional profiles reveal physiological mechanisms for compensation during a simulated marine heatwave in Yellowtail Kingfish (Seriola lalandi) SE Hook, RJ Farr, J Su, AJ Hobday, C Wingate,BMC genomics,2025
- ACOUSTIC EFFECTS ON JUVENILE FISH IN BREEDING: CASE OF STUDY OF DICENTRARCHUS LABRAX M Mauro, JR Soriano, V Espinosa, P Poveda,/,/
- Determination of stress levels of Otolithoides biauritus (Cantor, 1849) in selected zones of Indian Sundarbans: Stress levels of Otolithoides biauritus in Sunderbans J Mitra, S Mondal,Indian Journal of Experimental Biology,2024
- Growth, Nutrient Deposition, Plasma Metabolites, and Innate Immunity are Associated with Feeding Rate in Juvenile Starry Flounder (Platichthys Stellatus) JH Cho,/,/
- TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
- Cadmium induced oxidative stress, endoplasmic reticulum (ER) stress and apoptosis with compensative responses towards the up-regulation of ribosome, protein processing in the ER, and protein export pathways in the liver of zebrafish W Hu,Aquatic toxicology,2023
- Effects of black soldier fly (Hermetia illucens) pre-pupae meal on the growth, stress, and immune responses of juvenile rainbow trout (Oncorhynchus mykiss) reared at different stocking densities JH Cho,Aquaculture Reports,2022
- Effect of temperature on food consumption, immune system, antioxidant enzymes, and heat shock protein 70 of Channa punctata (Bloch, 1793). SP Singh,fish physiology and biochemistry,2020
- Effect of Light Intensity on Survival, Growth and Physiology of Rohu, Labeo rohita (Cyprinidae) Fry Khan N A, et al,International Journal of Radiation Biology,2019
- INVESTIGATING THE ROLE OF HEAT SHOCK PROTEINS AND MELANOPHORES DURING STRESS CAUSED BY HIGH LIGHT INTENSITY IN INDIAN MAJOR CARP, CATLA CATLA N. A. Khan, et al,International Journal of Pharmaceutical Sciences and Research,2018
- Dietary supplementation of vitamin C: an effective measure to give protection against UV-B irradiation using fish as model organism Rina Chakrabarti, et al,Photochemical & Photobiological Sciences,2018
- Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015
- Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice Wang YY. et al,Drug Des Devel Ther,2015
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