Fish Acetylcholinesterase(AChE)ELISA Kit

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中文名稱：

魚乙酰膽堿酯酶(AChE)酶聯免疫試劑盒
貨號：

CSB-E17001Fh
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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魚乙酰膽堿酯酶(AChE)酶聯免疫試劑盒（CSB-E17001Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AChE含量。AChE即乙酰膽堿酯酶，是一種重要的神經遞質水解酶。背景上，它在神經系統中對維持神經沖動正常傳導至關重要。研究機制方面，主要聚焦于其如何高效水解乙酰膽堿，以調控神經信號傳遞，相關研究可為神經系統疾病治療藥物研發提供方向。試劑盒檢測范圍為2.5 ng/mL-40 ng/mL，該產品適用于科研領域的環境毒理學研究，例如水體污染物對魚類神經功能的影響評估、生態毒性機制探究或實驗室條件下魚類模型的神經生化指標監測，為水產養殖安全、環境污染生物標志物篩選等研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
縮寫：

AChE
Uniprot No.：

P04058
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 ng/mL-40 ng/mL
靈敏度：

2.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	92
	Range %	87-101
1:4	Average %	93
	Range %	87-100
1:8	Average %	89
	Range %	83-99

回收率：

The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	87-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.284	0.292	0.288
20	0.521	0.532	0.527
10	0.886	0.891	0.889
5	1.374	1.382	1.378
2.5	2.583	2.599	2.591

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Positive Effects of Seabuckthorn oil on Scopolamine Induced PC12 Cell Damage and APP/PS1 Mice D Yang, S Zhang, D Wang, Q Yue,Natural Product Communications,2025
Triklorfonun Gammarus pulex'te Baz? Enzimatik Antioksidanlara Etkisinin De?erlendirilmesi ?T Yolcular,Ulusal ?evre Bilimleri Ara?t?rma Dergisi,/
Neurotoxic Effect of New Generation Insecticide with Flubendiamide Active Ingredient in Gammarus pulex (Linnaeus, 1758) (Amphipoda: Gammaridae) at Different Temperatures H S?ylemez,/,2023
Physiochemical properties and neuroprotective function of Korean major yuzu varieties BB LEE,Food Science and Technology,2023
Potential Adverse Outcome Pathway of Oxidative Stress Induction and Neurodevelopmental Toxicity Mediated by Three Alkyl Organophosphate Flame Retardants in Zebrafish Larvae CH Lee,/,2024
Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullan?larak Belirlenmesi NC Yildirim,researchgate,2022
Biomonitoring the effects of urban-stream waters on the health status of pale chub (Zacco platypus): A comparative analysis of biological indexes and biomarker levels CB Park,Ecotoxicology and Environmental Safety,2020
Exposure Through Runoff and Ground Water Contamination Differentially Impact Behavior and Physiology of Crustaceans in Fluvial Systems Alexandra N. Steele.et al,ARCH ENVIRON CON TOX,2018
Are mussels able to distinguish underwater sounds? Assessment of the reactions of Mytilus galloprovincialis after exposure to lab-generated acoustic signals. Vazzana M.et al,Comp Biochem Physiol A Mol Integr Physiol.,2016

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功能：

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
亞細胞定位：

[Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
蛋白家族：

Type-B carboxylesterase/lipase family
組織特異性：

Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.

Fish Acetylcholinesterase(AChE)ELISA Kit

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