Escherichia coli protein (E.coli P) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大腸桿菌宿主殘留蛋白(E.coli P)酶聯免疫試劑盒（CSB-E11306o）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的E.coli P含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可有效監測生物制品生產過程中的宿主蛋白殘留；滿足生物制藥工藝開發、重組蛋白純化效果驗證、轉基因生物樣本分析等科研場景；適用于實驗室規模的生產監控、生物工程研究中的質量評估以及基礎科研中的宿主蛋白殘留分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

E.coli P
種屬：

Escherichia Coli
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Microbiology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of escherichia coli protein in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of escherichia coli protein spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	96	89-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.