Escherichia coli protein (E.coli P) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大腸桿菌宿主殘留蛋白(E.coli P)酶聯(lián)免疫試劑盒（CSB-E11306o）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的E.coli P含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可有效監(jiān)測生物制品生產(chǎn)過程中的宿主蛋白殘留；滿足生物制藥工藝開發(fā)、重組蛋白純化效果驗證、轉(zhuǎn)基因生物樣本分析等科研場景；適用于實驗室規(guī)模的生產(chǎn)監(jiān)控、生物工程研究中的質(zhì)量評估以及基礎(chǔ)科研中的宿主蛋白殘留分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

E.coli P
種屬：

Escherichia Coli
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Microbiology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of escherichia coli protein in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of escherichia coli protein spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	96	89-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.