Dog von Willebrand Factor,vWF ELISA Kit

Dog von Willebrand Factor,vWF ELISA Kit

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中文名稱：

犬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E12759c
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
促銷：
其他：

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產(chǎn)品描述：

犬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒（CSB-E12759c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VWF含量。VWF即血管性血友病因子，其在止血過程中發(fā)揮關(guān)鍵作用。當(dāng)血管受損時(shí)，VWF能介導(dǎo)血小板黏附于受損血管壁。研究機(jī)制上多圍繞其結(jié)構(gòu)與功能關(guān)系，以及與其他凝血因子相互作用，目前它也是出血性和血栓性疾病研究的重要靶點(diǎn)。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于科研場(chǎng)景中犬類VWF蛋白表達(dá)水平的定量分析，可用于探究凝血相關(guān)疾病的分子機(jī)制、評(píng)估實(shí)驗(yàn)動(dòng)物模型的血栓或出血性病理狀態(tài)，以及篩選影響凝血功能的藥物或化合物研究，為獸醫(yī)基礎(chǔ)研究與生物醫(yī)學(xué)實(shí)驗(yàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
縮寫：

VWF
Uniprot No.：

Q28295
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.32 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	101
	Range %	95-107
1:200	Average %	94
	Range %	90-100
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

回收率：

The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.093	0.101	0.097
20	0.209	0.216	0.213
10	0.422	0.441	0.432
5	0.739	0.715	0.727
2.5	1.168	1.124	1.146
1.25	1.602	1.709	1.656
0.625	1.779	1.879	1.829
0	2.170	2.297	2.234

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
基因功能參考文獻(xiàn)：
1. only the c.1657G variant was found in the homozygous state exclusively in Von Willebrand disease affected dogs, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer and German Shorthaired Pointer breeds PMID: 28696025
2. the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus PMID: 17593818
3. Expression of mutant VWF proteins confirmed that VWF multimerization is not necessary for Weibel-Palade body creation PMID: 15331450
相關(guān)疾病：

Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF.
亞細(xì)胞定位：

Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
組織特異性：

Plasma.
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:399544

STRING: 9615.ENSCAFP00000022453

UniGene: Cfa.111

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.