Dog thrombomodulin,TM ELISA Kit

Dog thrombomodulin,TM ELISA Kit

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中文名稱：

犬血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06529c
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒（CSB-E06529c）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的THBD含量。THBD即凝血酶調(diào)節(jié)蛋白，它是血管內(nèi)皮細(xì)胞表面的凝血酶受體。其背景上與凝血、炎癥等密切相關(guān)。研究機(jī)制方面，它能和凝血酶結(jié)合，改變其底物特異性，激活蛋白C，進(jìn)而發(fā)揮抗凝、抗炎、保護(hù)血管內(nèi)皮等作用，在多種疾病研究中有重要意義。試劑盒檢測(cè)范圍為23.44 pg/mL-1500 pg/mL，是研究犬類凝血功能異常、心血管疾病及炎癥相關(guān)機(jī)制的重要生物標(biāo)志物；適用于科研領(lǐng)域中對(duì)犬類血栓形成機(jī)制、內(nèi)皮功能障礙及藥物干預(yù)效果的體外研究，為動(dòng)物模型實(shí)驗(yàn)提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

THBD ELISA Kit; Thrombomodulin ELISA Kit; TM ELISA Kit; CD antigen CD141 ELISA Kit
縮寫：

THBD
Uniprot No.：

Q5W7P8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

23.44 pg/mL-1500 pg/mL
靈敏度：

5.86 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	97
	Range %	91-103
1:20	Average %	94
	Range %	91-98
1:40	Average %	90
	Range %	88-94
1:80	Average %	93
	Range %	89-97

回收率：

The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	93	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1500	0.087	0.086	0.087
750	0.148	0.142	0.145
375	0.234	0.229	0.232
187.5	0.347	0.338	0.343
93.75	0.562	0.576	0.569
46.88	0.865	0.921	0.893
23.44	1.464	1.392	1.428
0	2.677	2.568	2.623

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
基因功能參考文獻(xiàn)：
1. plasma levels are higher in dogs infected with Leishmania infantum PMID: 15372989
2. cDNA sequence analysis, amino acid sequence homology, and tissue expression. PMID: 15585960
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Expressed in lung, liver, spleen, kidney, pancreas and lymph node. Low expression in heart, cerebrum, urinary bladder and uterus.
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:474355

STRING: 9615.ENSCAFP00000007702

UniGene: Cfa.16238

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.