Dog motilin (MLN) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬胃動素(MLN)酶聯(lián)免疫試劑盒（CSB-EL014638DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MLN含量。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，該產(chǎn)品適用于科研領(lǐng)域，例如在動物營養(yǎng)學(xué)實驗中評估飼料成分對胃腸激素分泌的影響，或在基礎(chǔ)醫(yī)學(xué)研究中探索胃腸功能紊亂的分子機制，為犬類消化生理及病理研究提供定量分析支持。研究犬消化系統(tǒng)疾病模型、胃腸動力調(diào)節(jié)機制或相關(guān)藥物干預(yù)實驗。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

MLN ELISA Kit; Motilin ELISA Kit
縮寫：

MLN
Uniprot No.：

P19863
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of dog MLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle.
亞細胞定位：

Secreted.
蛋白家族：

Motilin family
數(shù)據(jù)庫鏈接：

STRING: 9615.ENSCAFP00000001703