Dog S100 calcium binding protein B (S100B) ELISA kit
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中文名稱：犬S100B蛋白(S100B)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL020643DO
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬S100B蛋白(S100B)酶聯(lián)免疫試劑盒（CSB-EL020643DO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的S100B含量。S100B是一種鈣結(jié)合蛋白，主要存在于神經(jīng)膠質(zhì)細(xì)胞中。在神經(jīng)系統(tǒng)疾病如阿爾茨海默病、腦損傷中，S100B水平會(huì)出現(xiàn)異常。研究發(fā)現(xiàn)它可通過多種信號(hào)通路影響神經(jīng)細(xì)胞生長(zhǎng)、凋亡等，其異常表達(dá)可能參與疾病的發(fā)生發(fā)展。試劑盒檢測(cè)范圍為39 pg/mL-2500 pg/mL，該產(chǎn)品適用于神經(jīng)科學(xué)基礎(chǔ)研究、疾病模型評(píng)估及藥物開發(fā)等科研場(chǎng)景，例如通過檢測(cè)外周血或腦組織樣本中的S100B水平，探究犬類神經(jīng)退行性病變機(jī)制或創(chuàng)傷性腦損傷的生物標(biāo)志物動(dòng)態(tài)變化，為動(dòng)物實(shí)驗(yàn)提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：39 pg/mL-2500 pg/mL
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靈敏度：9.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-91 1:2 Average % 93 Range % 87-98 1:4 Average % 102 Range % 98-106 1:8 Average % 98 Range % 94-103 -
回收率：
The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-102 EDTA plasma (n=4) 89 84-95 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2500 1.955 2.021 1.988 1.816 1250 1.474 1.522 1.498 1.326 625 0.911 0.987 0.949 0.777 312.5 0.526 0.536 0.531 0.359 156.2 0.365 0.345 0.355 0.183 78.1 0.293 0.312 0.303 0.131 39 0.221 0.226 0.224 0.052 0 0.175 0.169 0.172 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
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