Dog Orexin(HCRT) ELISA kit

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產品詳情

產品描述：

犬阿立新(HCRT)酶聯免疫試劑盒（CSB-EL010230DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HCRT含量。HCRT（食欲素）是一種神經肽，在睡眠-覺醒周期調控等方面發揮重要作用。其背景方面與維持清醒狀態相關。研究機制上，它通過與特定受體結合，調節神經元活動，影響多種生理功能，對其研究有助于深入理解睡眠障礙等相關疾病。試劑盒檢測范圍為1.87 pg/mL-120 pg/mL，適用于神經生物學、動物行為學等領域的科學研究，例如探究犬類睡眠 - 覺醒周期調控機制、代謝疾病模型中的HCRT動態變化，或評估藥物干預對HCRT信號通路的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HCRT ELISA Kit; OX ELISA Kit; PPOX ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
縮寫：

HCRT
Uniprot No.：

Q9GLF6
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.87 pg/mL-120 pg/mL
靈敏度：

0.47 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog HCRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	101
	Range %	98-105
1:4	Average %	94
	Range %	89-98
1:8	Average %	103
	Range %	96-107

回收率：

The recovery of dog HCRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-95
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
120	2.176	2.234	2.205	2.101
60	1.659	1.765	1.712	1.608
30	1.193	1.223	1.208	1.104
15	0.793	0.776	0.785	0.681
7.5	0.492	0.483	0.488	0.384
3.75	0.341	0.356	0.349	0.245
1.87	0.242	0.249	0.246	0.142
0	0.103	0.104	0.104

功能：

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
基因功能參考文獻：
1. The presence of orexin A in the ovary either in dog or in cat is in agreement with previous data on the presence and role of orexins in the female genital system of other species. PMID: 25601132
亞細胞定位：

Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse.
蛋白家族：

Orexin family
數據庫鏈接：

KEGG: cfa:607641

STRING: 9615.ENSCAFP00000022949

UniGene: Cfa.24652