Dog Interleukin 10,IL-10 ELISA Kit

Dog Interleukin 10,IL-10 ELISA Kit

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評價(jià)

相關(guān)產(chǎn)品

相關(guān)問答

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號通路

技術(shù)信息

聯(lián)系信息

文獻(xiàn)引用 (1) 說明書下載

中文名稱：

犬白介素10(IL-10)酶聯(lián)免疫試劑盒
貨號：

CSB-E11261c
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
促銷：
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
相關(guān)問答
靶點(diǎn)詳情

產(chǎn)品描述：

犬白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E11261c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種具有重要生物學(xué)意義的靶點(diǎn)。它是免疫調(diào)節(jié)細(xì)胞因子，具有抗炎特性。研究機(jī)制上，IL10可抑制促炎細(xì)胞因子產(chǎn)生，調(diào)節(jié)免疫細(xì)胞功能。對其深入研究有助于理解免疫相關(guān)疾病發(fā)病機(jī)制，為開發(fā)新治療方法提供方向。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于犬類免疫機(jī)制研究、炎癥性疾病模型評估、疫苗或藥物干預(yù)效果分析等科研場景，為探索IL - 10在感染、腫瘤或自身免疫性疾病中的調(diào)控機(jī)制提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
縮寫：

IL10
Uniprot No.：

P48411
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-108
1:2	Average %	97
	Range %	91-105
1:4	Average %	89
	Range %	84-96
1:8	Average %	92
	Range %	87-98

回收率：

The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	92	87-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.954	2.967	2.961	2.853
50	2.388	2.398	2.393	2.285
25	1.758	1.786	1.772	1.664
12.5	1.035	1.109	1.072	0.964
6.25	0.502	0.546	0.524	0.416
3.12	0.271	0.267	0.269	0.161
1.56	0.184	0.196	0.190	0.082
0	0.112	0.103	0.108

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The Targeted Regulation of Canine Optic Nerve CB2R Affects the Direction of Microglial Cell Differentiation Ye Zi, et al,Toxicon,2019

功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
基因功能參考文獻(xiàn)：
1. seven new genetic variations of the IL10 gene were identified. PMID: 24487092
2. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
3. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-10 family
數(shù)據(jù)庫鏈接：

KEGG: cfa:403628

STRING: 9615.ENSCAFP00000016812

UniGene: Cfa.38

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.