Dog Hepcidin(HAMP) ELISA kit

說明書下載

中文名稱：

犬鐵調素(HAMP)酶聯免疫試劑盒
貨號：

CSB-EL010124DO
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬鐵調素(HAMP)酶聯免疫試劑盒（CSB-EL010124DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的HAMP含量。HAMP即肝臟抗菌肽，其背景在于它是肝臟產生的一種關鍵抗菌物質，參與鐵代謝和免疫調節。研究機制上，它能結合并降解鐵轉運蛋白，減少細胞鐵外流；還可作為先天免疫效應分子，抵御病原體入侵，在維持機體鐵穩態與抗感染中起重要作用。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，支持科研人員探究犬類鐵代謝相關疾病的分子機制，例如在鐵負荷異常模型、營養干預實驗或遺傳性鐵代謝紊亂研究中的應用。實驗結果可助力動物生理學、病理學及藥理學等領域的基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HAMP ELISA Kit; Hepcidin ELISA Kit
縮寫：

HAMP
Uniprot No.：

Q5U9D2
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	96-111
1:2	Average %	100
	Range %	90-104
1:4	Average %	97
	Range %	90-101
1:8	Average %	91
	Range %	82-95

回收率：

The recovery of dog HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	107	99-111

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	1.971	1.912	1.942	1.810
100	1.482	1.477	1.480	1.348
50	0.976	0.988	0.982	0.850
25	0.546	0.556	0.551	0.419
12.5	0.402	0.378	0.390	0.258
6.25	0.263	0.259	0.261	0.129
3.12	0.178	0.172	0.175	0.043
0	0.131	0.133	0.132

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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■ 常見問題解答

My name is Thitapa, student of veterinary medicine, Chulalongkorn university from Thailand.
Recently we are working on a study about canine hepcidin and I would like to know how much it is for 1 dog hepcidin ELISA test kit 96-well (I couldn't find it on the website ,only found the 24-T one) Moreover, if you have any evidence about any studies that your product had been used to evaluate hepcidin level please attach the information. We would be really appreciated.

Thanks for your concern on Cusabio and your email!We can supply both size of Dog Hepcidin(HAMP) ELISA kit ,24T & 96T.
#CSB-EL010124DO,Dog Hepcidin(HAMP) ELISA kit -96T
Regarding this kit,we tested the normal dog serum ,plasma samples with 0 dilutions and we got the results falling in 12.5--100ng/ml.You can take this for your reference.As long as the content of the sample falls in the detection range of this kit,it can be detected out.And I will send you manual reference by email later.
If you are interested to order it,pls kindly contact and enquire local distributor.

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功能：

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
基因功能參考文獻：
1. The objectives of this study were to clone and sequence the canine hepcidin gene and to gather preliminary data about tissue expression of hepcidin in dogs. PMID: 15570559
亞細胞定位：

Secreted.
蛋白家族：

Hepcidin family
數據庫鏈接：

KEGG: cfa:492281

STRING: 9615.ENSCAFP00000010474

UniGene: Cfa.14486

Dog Hepcidin(HAMP) ELISA kit

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