Dog Erythropoietin,EPO ELISA Kit

說明書下載

產品詳情

產品描述：

犬紅細胞生成素(EPO)酶聯免疫試劑盒（CSB-E11303c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPO含量。EPO即促紅細胞生成素，是一種糖蛋白激素，由腎臟分泌。其背景在于它能促進紅細胞生成，維持機體正常的氧運輸能力。研究機制方面，EPO作用于骨髓中紅系祖細胞表面受體，促進紅系祖細胞增殖、分化和成熟，增加紅細胞數量，在治療貧血等方面有重要意義。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，適用于科研場景中犬類貧血機制研究、腎臟功能與造血系統關聯性分析、藥物干預效果評價或疾病模型（如腎衰竭、腫瘤性貧血）的EPO動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EPOErythropoietin ELISA kit
縮寫：

EPO
Uniprot No.：

P33707
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-105
1:2	Average %	89
	Range %	80-97
1:4	Average %	93
	Range %	86-100
1:8	Average %	95
	Range %	88-100

回收率：

The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	83-102
EDTA plasma (n=4)	95	85-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
基因功能參考文獻：
1. Developmental and compensatory lung growth involve paracrine erythropoietin signaling with parallel upregulation but differential processing of the erythropoietin receptor. PMID: 15286000
2. apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts PMID: 15916748
亞細胞定位：

Secreted.
蛋白家族：

EPO/TPO family
組織特異性：

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
數據庫鏈接：

KEGG: cfa:404002

STRING: 9615.ENSCAFP00000038507

UniGene: Cfa.3871