Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

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中文名稱：

鹿胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒
貨號：

CSB-E12644De
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

鹿胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E12644De）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1（胰島素樣生長因子-1）是一種由IGF1基因編碼的蛋白質(zhì)，與胰島素具有相似的結(jié)構(gòu)和功能。它主要在肝臟合成，并通過生長激素（GH）的調(diào)節(jié)促進(jìn)生長和發(fā)育。IGF-1通過激活I(lǐng)GF-1受體，在多個組織和器官中調(diào)節(jié)細(xì)胞增殖、分化和凋亡。其研究機(jī)制涉及IGF-1與受體的結(jié)合，以及通過多種信號通路（如PI3K/AKT和MEK/ERK）影響細(xì)胞功能。試劑盒檢測范圍為1 ng/mL-200 ng/mL，科研人員可將其應(yīng)用于鹿科動物生長發(fā)育研究、營養(yǎng)干預(yù)實驗、繁殖生物學(xué)分析以及代謝相關(guān)疾病模型的機(jī)制探索，例如評估不同飼養(yǎng)條件下IGF - 1的動態(tài)變化，或探究其在組織再生修復(fù)中的作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IGF-1
Uniprot No.：

H9BDM1
種屬：

Deer
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1 ng/mL-200 ng/mL
靈敏度：

0.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	95
	Range %	89-104
1:4	Average %	99
	Range %	87-107
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.175	0.174	0.175
50	0.333	0.316	0.325
16	0.622	0.640	0.631
4	1.040	1.048	1.044
1	1.416	1.389	1.403
0	2.577	2.633	2.605

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections M López-Pedrouso,Biology,2021
Reproductive responses to sexually active buck of does treated with melatonin when body weight/body condition is increasing or decreasing Gallego-Calvo, L.et al, Animal Reproduction Science,2018
Detection and quantitation of insulin-like growth factor-1 in velvet antler by an enzyme-linked immunosorbent assay /,Consumer Electronics, Communications and Networks (CECNet),2011

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.