Chicken Tumor necrosis factor α,TNF-α ELISA KIT

Chicken Tumor necrosis factor α,TNF-α ELISA KIT

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中文名稱：

雞腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E11231Ch
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

雞腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E11231Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF-α含量。TNF-α（腫瘤壞死因子-α）是一種多功能促炎細胞因子，主要在免疫應答、炎癥反應和細胞凋亡中發揮作用。其研究機制涉及與受體TNFR1結合，激活多種信號通路，如線粒體途徑、Bcl-2家族蛋白、活性氧等，導致細胞凋亡和炎癥反應。TNF-α已成為治療自身免疫疾病的關鍵靶點。試劑盒檢測范圍為0.27 pg/mL-200 pg/mL，適用于科研領域研究禽類傳染性疾病（如禽流感、新城疫）的炎癥反應機制，評估疫苗或抗炎藥物對免疫系統的調控效果，以及構建家禽疾病模型時監測炎癥因子動態變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
縮寫：

TNF-α
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.27 pg/mL-200 pg/mL
靈敏度：

0.27 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	100
	Range %	95-105
1:4	Average %	87
	Range %	82-91
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-108
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.173	0.170	0.172
66.7	0.259	0.251	0.255
22.2	0.347	0.367	0.357
7.41	0.452	0.488	0.470
2.47	0.611	0.633	0.622
0.82	0.809	0.855	0.832
0.27	1.033	1.018	1.026
0	1.748	1.707	1.728

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Effects of dietary Bacillus velezensis fermented soybean hull supplementation on antioxidant capacity, suppressing pro-inflammatory, and modulating microbiota composition in broilers YH Chen, YC Li, SC Chang, MJ Lin, LJ Lin, TT Lee,Poultry Science,2025
Effect of Bile Acids Supplementation in Fatty Liver Hemorrhagic Syndrome, Production Performance, Physiological and Quality Characteristics of Laying Hen Eggs W Li,Animals,2024
Rutin Nanoparticles Alleviate Cadmium-Induced Oxidative and Immune Damage in Broilers’ Bursa of Fabricius via Modulating Hsp70/TLR4/NF-ΚB Signaling Pathway M Abomosallam,Biological trace element research,2024
Serum inflammation and oxidative DNA damage amelioration in cocks fed supplemental Vernonia amygdalina and zinc in aflatoxin B1 contaminated diets OJ Olarotimi,Translational Animal Science,2023
Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B1 contaminated diets OJ Olarotimi,Olarotimi O J,,2023
Dual-Drug Loaded Separable Microneedles for Efficient Rheumatoid Arthritis Therapy M An,Pharmaceutics,2022
Influence of Dietary Supplementation of Guava Leaf, Oxytetracycline, and Tert-Butylhydroxytoluene on Growth Performance, Gut Microbial Population, Immune?… KD Adeyemi,Islamic Azad University,2022
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Impacts of dietary supplementation with nano-iron and methionine on growth, blood chemistry, liver biomarkers, and tissue histology of heat-stressed broiler chickens HG Abdel-Rahman,Trop Anim Health Prod,2022
Effects of essential oil/palygorskite composite on performance, egg quality, plasma biochemistry, oxidation status, immune response and intestinal morphology of laying hens H.Cheng,Poultry Science,2021
Hydroxy-Selenomethionine Improves the Selenium Status and Helps to Maintain Broiler Performances under a High Stocking Density and Heat Stress Conditions through a Better Redox and Immune Response H Sun,Antioxidants,2021
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Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
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最新研究進展：

TNF-α（腫瘤壞死因子-α）是一種促炎癥蛋白質，它在多種疾病中扮演重要角色。最新研究表明，TNF-α可能參與COVID-19的發病機制，因為這種病毒通過激活NF-κB途徑來增加TNF-α的表達，從而引發炎癥反應和危及生命的并發癥。此外，TNF-α也參與了許多自身免疫疾病的發生和發展過程，例如風濕性關節炎和腸道炎癥性疾病。

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.