Chicken Transforming Growth factor β1,TGF-β1 ELISA kit

Chicken Transforming Growth factor β1,TGF-β1 ELISA kit

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)產(chǎn)品

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號(hào)通路

技術(shù)信息

聯(lián)系信息

文獻(xiàn)引用 (9) 說(shuō)明書下載

中文名稱：

雞轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09875Ch
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品描述：

雞轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)酶聯(lián)免疫試劑盒（CSB-E09875Ch）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TGFB1含量。TGFB1是重要的靶點(diǎn)，它是一種多功能細(xì)胞因子。在細(xì)胞生長(zhǎng)、分化、凋亡等過(guò)程中發(fā)揮關(guān)鍵作用。其異常表達(dá)與多種疾病如腫瘤、纖維化等密切相關(guān)。研究機(jī)制主要圍繞其信號(hào)通路展開，旨在調(diào)控其活性來(lái)治療相關(guān)疾病。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于家禽免疫學(xué)基礎(chǔ)研究、病理模型建立及生物標(biāo)志物分析，特別在探究禽類傳染性疾病、疫苗效價(jià)評(píng)估或應(yīng)激反應(yīng)機(jī)制等科研場(chǎng)景中，能夠?yàn)閷?shí)驗(yàn)提供穩(wěn)定可靠的定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
縮寫：

TGFB1
Uniprot No.：

P09531
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	84-97
1:2	Average %	95
	Range %	84-98
1:4	Average %	96
	Range %	89-103
1:8	Average %	95
	Range %	85-100

回收率：

The recovery of chicken TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-97
EDTA plasma (n=4)	97	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.011	2.105	2.058	1.965
10	1.790	1.828	1.809	1.716
5	1.466	1.527	1.497	1.404
2.5	1.149	1.163	1.156	1.063
1.25	0.795	0.854	0.825	0.732
0.625	0.433	0.425	0.429	0.336
0.312	0.264	0.255	0.260	0.167
0	0.095	0.091	0.093

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella Huanzhi Zhao, et al,Parasitology Research,2019
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,The Thai Journal of Veterinary Medicine,2016
The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
Effect of garlic component s-allyl cysteine sulfoxide onglycatedhumanserum albumininduced activationof endothelial cells: aninvitro study B.K. NIRALA. et al,European Review for Medical and Pharmacological Sciences,2015

收起更多

功能：

Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
亞細(xì)胞定位：

[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
蛋白家族：

TGF-beta family
數(shù)據(jù)庫(kù)鏈接：

UniGene: Gga.929

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.