Chicken Cluster of Differentiation 4(CD4) ELISA Kit

Chicken Cluster of Differentiation 4(CD4) ELISA Kit

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中文名稱：

雞CD4分子(CD4)酶聯(lián)免疫試劑盒
貨號：

CSB-E13114C
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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雞CD4分子(CD4)酶聯(lián)免疫試劑盒（CSB-E13114C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD4含量。CD4是一種T細胞受體共受體，位于T細胞表面，與MHC II類分子結合，協(xié)助T細胞識別抗原。CD4在HIV感染中起關鍵作用，HIV病毒通過結合CD4進入T細胞，導致CD4+T細胞數(shù)量減少，免疫功能受損。研究CD4的功能和調(diào)控機制對于理解HIV感染和開發(fā)抗病毒藥物具有重要意義。試劑盒檢測范圍為3.125 ng/mL-800 ng/mL，適用于科研領域中對雞免疫系統(tǒng)的功能研究，包括感染性疾病模型中的T細胞應答分析、疫苗免疫效果評估以及免疫抑制性疾病機制探索等基礎研究，為禽類免疫學實驗提供可靠的定量檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CD4
Uniprot No.：

Q9W6V7
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.125 ng/mL-800 ng/mL
靈敏度：

3.125 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	91
	Range %	87-96
1:4	Average %	92
	Range %	87-98
1:8	Average %	101
	Range %	97-105

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

800

0.174

0.172

0.173

200

0.291

0.275

0.283

0.508

0.546

0.527

12.5

0.813

0.835

0.824

3.125

1.365

1.398

1.382

2.105

2.168

2.137

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

DETERMINATION OF DOPAMINE AND SEROTONIN WITH IMMUNE STATE CD4 AND CD8 OF THE NEWCASTLE INFECTION IN BROILERS AH Ulaiwi?,Iraqi Journal of Agricultural Sciences,2023
Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella Huanzhi Zhao, et al,Parasitology Research,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.