Canine connective tissue growth factor,CTGF ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬結(jié)締組織生長(zhǎng)因子(CTGF)酶聯(lián)免疫試劑盒（CSB-E13386c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CTGF含量。CTGF即結(jié)締組織生長(zhǎng)因子，是轉(zhuǎn)化生長(zhǎng)因子-β下游的促纖維化介質(zhì)。它在多種纖維化疾病發(fā)生發(fā)展中起關(guān)鍵作用。研究機(jī)制上，其能促進(jìn)細(xì)胞黏附、增殖、遷移和細(xì)胞外基質(zhì)合成等，所以是抗纖維化治療極具潛力的靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于犬類纖維化疾病模型研究、器官損傷修復(fù)機(jī)制探索以及相關(guān)代謝性疾病的分子機(jī)制分析等科研場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

CTGF
Uniprot No.：

J9NTX8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	93-101
1:4	Average %	85
	Range %	81-89
1:8	Average %	92
	Range %	85-97

回收率：

The recovery of canine CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	108	103-111

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.