Canine brain natriuretic peptide(BNP)ELISA Kit

Canine brain natriuretic peptide(BNP)ELISA Kit

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中文名稱(chēng)：

犬腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E17436c
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬腦鈉素/腦鈉尿肽(BNP)酶聯(lián)免疫試劑盒（CSB-E17436c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的BNP含量。BNP即腦鈉肽，主要由心室肌細(xì)胞分泌。它是反映心室功能的敏感指標(biāo)，在心室擴(kuò)張和壓力負(fù)荷增加時(shí)分泌增多。研究機(jī)制上，其可利鈉、利尿、舒張血管、降低血壓，抑制腎素-血管緊張素-醛固酮系統(tǒng)等，在心血管疾病評(píng)估等方面有重要價(jià)值。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，該產(chǎn)品適用于心血管疾病動(dòng)物模型研究、藥物干預(yù)效果評(píng)估或病理機(jī)制探索等科研領(lǐng)域，為研究者提供可靠的BNP濃度檢測(cè)方案，助力于犬類(lèi)心臟相關(guān)疾病的分子機(jī)制解析及生物標(biāo)志物研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 34 ELISA kit; BNP-34); Brain natriuretic peptide 29 ELISA kit; BNP-29)] ELISA kit
縮寫(xiě)：

BNP
Uniprot No.：

P16859
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	91
	Range %	88-96
1:4	Average %	95
	Range %	90-102
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-105
EDTA plasma (n=4)	96	92-101

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.867	1.953	1.910	1.706
400	1.524	1.600	1.562	1.358
200	1.166	1.124	1.145	0.941
100	0.812	0.862	0.837	0.633
50	0.524	0.563	0.544	0.340
25	0.375	0.401	0.388	0.184
12.5	0.278	0.259	0.269	0.065
0	0.203	0.204	0.204

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
亞細(xì)胞定位：

Secreted.; [Brain natriuretic peptide 32]: Secreted.
蛋白家族：

Natriuretic peptide family
組織特異性：

Brain and also in atria, but at much lower levels than ANP.
數(shù)據(jù)庫(kù)鏈接：

STRING: 9615.ENSCAFP00000024316

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.