Canine Relaxin(RLN) ELISA Kit

說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

犬松弛肽/松弛素(RLN)酶聯(lián)免疫試劑盒（CSB-E14982c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的RLN含量。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，適用于犬類生殖生物學(xué)研究、妊娠相關(guān)疾病模型構(gòu)建、心血管調(diào)節(jié)機(jī)制探索等科研領(lǐng)域，為動(dòng)物生殖健康、病理生理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

RLN ELISA Kit; Prorelaxin [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
縮寫：

RLN
Uniprot No.：

Q9TRM8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine RLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	95
	Range %	91-99
1:4	Average %	88
	Range %	84-93
1:8	Average %	97
	Range %	93-100

回收率：

The recovery of canine RLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-107
EDTA plasma (n=4)	99	94-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
基因功能參考文獻(xiàn)：
1. the luteal expression and localization of the RLN system was investigated by immunohistochemistry using custom-made antibodies and semi-quantitative PCR, at selected time points during gestation PMID: 29364921
2. The blood levels of multiple steroids and other hormones in adult male dogs with or without prostatic hyperplasia are reported. PMID: 23279510
3. The expression of relaxin and its receptors in canine mammary neoplasm tissue were studied, indicating a possible significant role of relaxin as an inducer of connective tissue remodelling. PMID: 19754574
亞細(xì)胞定位：

Secreted.
蛋白家族：

Insulin family
組織特異性：

Placenta; syncytiotrophoblast.
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:403742

STRING: 9615.ENSCAFP00000031075

UniGene: Cfa.3608