Canine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Canine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

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中文名稱：

犬催乳素(PRL/LTH)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14930c
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬催乳素(PRL/LTH)酶聯(lián)免疫試劑盒（CSB-E14930c）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PRL/LTH含量。PRL即催乳素，LTH即促乳素，二者本質(zhì)相同。此靶點(diǎn)與乳腺發(fā)育及泌乳等生理過程密切相關(guān)。研究機(jī)制上，聚焦于其分泌調(diào)控、信號(hào)傳導(dǎo)通路等，旨在明確異常水平與疾病關(guān)聯(lián)，為高泌乳素血癥等疾病診療提供依據(jù)。試劑盒檢測(cè)范圍為7.5 μIU/mL-3000 μIU/mL，為科研人員研究犬類生殖生理、泌乳機(jī)制及內(nèi)分泌疾病模型提供可靠工具。特別適用于動(dòng)物繁殖生物學(xué)、激素調(diào)控機(jī)制探索以及藥物干預(yù)對(duì)泌乳功能影響等相關(guān)領(lǐng)域的研究，其兼容血清、血漿及多類型組織樣本的特點(diǎn)，可滿足實(shí)驗(yàn)設(shè)計(jì)中跨組織位點(diǎn)的激素水平對(duì)比分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PRL/LTH
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

7.5 μIU/mL-3000 μIU/mL
靈敏度：

7.5 μIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	91-100
1:2	Average %	90
	Range %	83-104
1:4	Average %	92
	Range %	89-100
1:8	Average %	96
	Range %	91-105

回收率：

The recovery of canine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	94	89-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
3000	0.405	0.408	0.407
562.5	0.733	0.726	0.730
93.75	1.198	1.195	1.197
26.25	1.479	1.484	1.482
7.5	1.621	1.626	1.624
0	2.445	2.446	2.446

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.