Canine Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬基質金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯免疫試劑盒（CSB-E14056c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基質金屬蛋白酶 2，在細胞外基質降解和重構中起關鍵作用。其異常表達與腫瘤生長、侵襲、轉移等密切相關。研究主要圍繞其表達調控機制、與其他分子相互作用等，以探索開發針對它的治療癌癥等疾病的藥物。試劑盒檢測范圍為4.69 ng/mL-300 ng/mL，適用于犬類疾病模型研究中MMP-2動態變化的監測，如腫瘤侵襲轉移機制分析、傷口愈合評估或心血管疾病相關基質重塑研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MMP2/Gelatinase A
Uniprot No.：

F1PMK7
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.69 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	85
	Range %	80-95
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.