Canine Matrix metalloproteinase 13,MMP-13 ELISA kit
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中文名稱(chēng)：犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E14055c
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14055c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP-13含量。MMP-13即基質(zhì)金屬蛋白酶13，在人體組織重塑、炎癥反應(yīng)等生理過(guò)程中起重要作用。異常表達(dá)與多種病理情況相關(guān)，如關(guān)節(jié)炎、癌癥等。研究主要圍繞其酶活性調(diào)控機(jī)制，旨在開(kāi)發(fā)針對(duì)此靶點(diǎn)的藥物，以干預(yù)相關(guān)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域?qū)θ?lèi)骨關(guān)節(jié)疾病模型、軟骨退行性病變機(jī)制以及抗炎藥物研發(fā)等研究方向的樣本檢測(cè)需求；滿足實(shí)驗(yàn)室對(duì)犬MMP - 13蛋白表達(dá)水平的高通量檢測(cè)及動(dòng)態(tài)監(jiān)測(cè)要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：MMP-13
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類(lèi)型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cancer
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 88-99 1:2 Average % 99 Range % 95-106 1:4 Average % 87 Range % 84-92 1:8 Average % 91 Range % 85-98 -
回收率：
The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-95 EDTA plasma (n=4) 88 85-92 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 3.160 3.255 3.208 3.107 5 2.614 2.712 2.663 2.562 2.5 1.784 1.932 1.858 1.757 1.25 1.026 1.030 1.028 0.927 0.625 0.478 0.487 0.483 0.382 0.312 0.264 0.256 0.260 0.159 0.156 0.156 0.166 0.161 0.060 0 0.105 0.097 0.101 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018
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