Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

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中文名稱：

犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14055c
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
促銷：
其他：

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產(chǎn)品描述：

犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14055c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP-13含量。MMP-13即基質(zhì)金屬蛋白酶13，在人體組織重塑、炎癥反應(yīng)等生理過(guò)程中起重要作用。異常表達(dá)與多種病理情況相關(guān)，如關(guān)節(jié)炎、癌癥等。研究主要圍繞其酶活性調(diào)控機(jī)制，旨在開(kāi)發(fā)針對(duì)此靶點(diǎn)的藥物，以干預(yù)相關(guān)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域?qū)θ惞顷P(guān)節(jié)疾病模型、軟骨退行性病變機(jī)制以及抗炎藥物研發(fā)等研究方向的樣本檢測(cè)需求；滿足實(shí)驗(yàn)室對(duì)犬MMP - 13蛋白表達(dá)水平的高通量檢測(cè)及動(dòng)態(tài)監(jiān)測(cè)要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

MMP-13
Uniprot No.：

F1PVS9
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	99
	Range %	95-106
1:4	Average %	87
	Range %	84-92
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	88	85-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	3.160	3.255	3.208	3.107
5	2.614	2.712	2.663	2.562
2.5	1.784	1.932	1.858	1.757
1.25	1.026	1.030	1.028	0.927
0.625	0.478	0.487	0.483	0.382
0.312	0.264	0.256	0.260	0.159
0.156	0.156	0.166	0.161	0.060
0	0.105	0.097	0.101

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.