Canine Interferon alpha-1/2 ELISA kit

Canine Interferon alpha-1/2 ELISA kit

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中文名稱(chēng)：

犬α-1/2干擾素(IFN-α1/2)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09849c
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬α-1/2干擾素(IFN-α1/2)酶聯(lián)免疫試劑盒（CSB-E09849c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IFN-A1/2含量。IFN-A1/2屬干擾素家族，在機(jī)體免疫防御中起關(guān)鍵作用。研究發(fā)現(xiàn)其能激活細(xì)胞內(nèi)信號(hào)通路，誘導(dǎo)抗病毒蛋白表達(dá)以抵御病毒感染。還參與調(diào)節(jié)免疫細(xì)胞活性和功能，在對(duì)抗腫瘤及自身免疫病中展現(xiàn)潛在價(jià)值，是研究熱點(diǎn)。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，適用于科研場(chǎng)景中犬類(lèi)I型干擾素的功能研究；其動(dòng)態(tài)水平變化常被用于評(píng)估病毒感染模型、疫苗免疫原性實(shí)驗(yàn)或免疫調(diào)節(jié)相關(guān)機(jī)制研究；細(xì)胞培養(yǎng)上清液可分析體外刺激后免疫細(xì)胞分泌情況，組織勻漿則適用于疾病模型中局部干擾素水平的定量分析；為動(dòng)物免疫學(xué)、病毒學(xué)及炎癥機(jī)制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
縮寫(xiě)：

IFN-α1/2
Uniprot No.：

P81255
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類(lèi)型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	100
	Range %	94-109
1:4	Average %	92
	Range %	89-96
1:8	Average %	87
	Range %	83-96

回收率：

The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	88-99

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.815	2.824	2.820	2.726
400	2.417	2.425	2.421	2.327
200	1.834	1.863	1.849	1.755
100	1.042	1.112	1.077	0.983
50	0.518	0.563	0.541	0.447
25	0.248	0.269	0.259	0.165
12.5	0.126	0.131	0.129	0.035
0	0.091	0.096	0.094

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:100685213

UniGene: Cfa.4550

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.