Canine Free Thyroxine,FT4 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

犬游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒（CSB-E13196c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FT4含量。FT4即游離甲狀腺素，是甲狀腺功能檢查的重要靶點。它能直接反映甲狀腺功能狀態(tài)。研究機制上，主要通過檢測其在血液中的含量，輔助醫(yī)生判斷甲狀腺疾病，如甲亢時FT4水平升高，甲減時則降低。試劑盒檢測范圍為7.5 ng/mL-120 ng/mL，適用于科研領(lǐng)域中對犬類甲狀腺功能的機制研究、疾病模型評估、營養(yǎng)代謝實驗或藥理學(xué)研究，能夠為動物醫(yī)學(xué)、比較內(nèi)分泌學(xué)等基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FT4
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.5 ng/mL-120 ng/mL
靈敏度：

7.5 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-97
1:2	Average %	90
	Range %	88-100
1:4	Average %	95
	Range %	90-105
1:8	Average %	91
	Range %	85-102

回收率：

The recovery of canine FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-105
EDTA plasma (n=4)	95	89-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.