Canine Dihydrotestosterone,DHT ELISA Kit

Canine Dihydrotestosterone,DHT ELISA Kit

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中文名稱：

犬雙氫睪酮(DHT)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E07881c
規(guī)格：

96T/48T
價(jià)格：

￥4800/￥3000
促銷：
其他：

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產(chǎn)品描述：

犬雙氫睪酮(DHT)酶聯(lián)免疫試劑盒（CSB-E07881c）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的DHT含量。DHT即雙氫睪酮，是睪酮在5α-還原酶作用下轉(zhuǎn)化而來(lái)。它與雄激素性脫發(fā)、前列腺增生等疾病密切相關(guān)。研究機(jī)制方面，主要聚焦其和靶細(xì)胞內(nèi)雄激素受體結(jié)合，影響基因表達(dá)，促進(jìn)細(xì)胞生長(zhǎng)分化，目前也是相關(guān)疾病藥物研發(fā)重要靶點(diǎn)。試劑盒檢測(cè)范圍為40 pg/mL-8000 pg/mL，該產(chǎn)品適用于動(dòng)物實(shí)驗(yàn)中對(duì)激素干預(yù)效果評(píng)估、生殖健康研究或代謝性疾病模型的 DHT 動(dòng)態(tài)分析，例如前列腺增生、脫發(fā)機(jī)制或內(nèi)分泌紊亂相關(guān)研究，為探索犬類生理病理機(jī)制提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫：

DHT
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

40 pg/mL-8000 pg/mL
靈敏度：

20 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-104
1:8	Average %	89
	Range %	82-95

回收率：

The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	99	98-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
8000	0.224	0.223	0.224
2000	0.362	0.356	0.359
640	0.564	0.557	0.561
160	0.819	0.804	0.812
40	1.174	1.275	1.225
0	2.305	2.404	2.355

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Finasteride accelerates prostate wound healing after thulium laser resection through DHT and AR signalling Zhao R.et al,Cell Prolif,2017
Androgen Deprivation Accelerates the Prostatic Urethra Wound Healing After Thulium Laser Resection of the Prostate by Promoting Re-Epithelialization and Regulating the Macrophage Polarization Xing-Jie Wang.et al,THE PROSTATE,2017

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.